Cell-surface Proteoglycan Expression by Lymphocytes from Peripheral Blood and Gingiva in Health and Periodontal Disease

Cell-surface proteoglycans are involved in lymphocyte migration and activation. This study investigated the expression of syndecan-1, syndecan-4, and glypican in peripheral blood lymphocytes and by lymphocytes in variously inflamed periodontal tissues. Gingival specimens from healthy, gingivitis, or...

Full description

Saved in:
Bibliographic Details
Published in:Journal of dental research Vol. 80; no. 8; pp. 1704 - 1710
Main Authors: Manakil, J.F., Sugerman, P.B., Li, H., Seymour, G.J., Bartold, P.M.
Format: Journal Article
Language:English
Published: Los Angeles, CA SAGE Publications 01.08.2001
SAGE PUBLICATIONS, INC
Subjects:
Adult
Aged
Alveolar Bone Loss - metabolism
Alveolar Bone Loss - pathology
Analysis of Variance
B-Lymphocytes - metabolism
B-Lymphocytes - pathology
cell-surface proteoglycans
Chronic Disease
Female
Flow Cytometry
Gingiva - metabolism
Gingiva - pathology
Gingival Hemorrhage - metabolism
Gingival Hemorrhage - pathology
Gingivitis - blood
Gingivitis - metabolism
Gingivitis - pathology
Growth Substances - pharmacology
Heparan Sulfate Proteoglycans - analysis
Humans
lymphocytes
Lymphocytes - metabolism
Lymphocytes - pathology
Macrophages - metabolism
Macrophages - pathology
Male
Membrane Glycoproteins - analysis
Middle Aged
Mitogens - pharmacology
Periodontal Attachment Loss - metabolism
Periodontal Attachment Loss - pathology
periodontal disease
Periodontal Pocket - metabolism
Periodontal Pocket - pathology
Periodontitis - blood
Periodontitis - metabolism
Periodontitis - pathology
Plasma Cells - metabolism
Plasma Cells - pathology
Proteoglycans - analysis
Regression Analysis
Statistics as Topic
Syndecan-1
Syndecan-4
Syndecans
T-Lymphocytes - metabolism
T-Lymphocytes - pathology
Tooth Cervix - pathology
periodontal disease
lymphocytes
cell-surface proteoglycans
ISSN:0022-0345, 1544-0591
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell-surface proteoglycans are involved in lymphocyte migration and activation. This study investigated the expression of syndecan-1, syndecan-4, and glypican in peripheral blood lymphocytes and by lymphocytes in variously inflamed periodontal tissues. Gingival specimens from healthy, gingivitis, or chronic periodontitis sites were stained by means of antibodies against B- and T-lymphocytes and also syndecan-1, syndecan-4, and glypican. Syndecan-1 expression by peripheral blood mononuclear cells (PBMC) from healthy, gingivitis, and chronic periodontitis subjects was assessed by flow cytometry. Syndecan-1 was expressed by B-cells/plasma cells but not T-cells in both gingivitis and chronic periodontitis lesions. Both B-cells/plasma cells and T-cells in gingivitis and chronic periodontitis expressed syndecan-4. Glypican was expressed only by macrophages. Stimulation of PBMC with mitogens and growth factors modulated syndecan-1 expression in both the T- and B-cells. Thus, cell-surface proteoglycan expression by lymphocytes in periodontal inflammation is cell-type-specific and may be modulated by inflammation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:0022-0345
1544-0591
DOI:10.1177/00220345010800080501