Isolation and characterization of exosomes released from mosquito cells infected with dengue virus

•Exosomes from mosquito cells contain proteins homologs to human tetraspanins.•Exosomes from dengue virus-infected mosquito cells are larger than the ones from uninfected cells.•Exosomes from dengue virus-infected mosquito cells can infect naïve mosquito cells.•Exosomes play a role in virus dissemin...

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Vydáno v:Virus research Ročník 266; s. 1 - 14
Hlavní autoři: Reyes-Ruiz, José Manuel, Osuna-Ramos, Juan Fidel, De Jesús-González, Luis Adrián, Hurtado-Monzón, Arianna Mahely, Farfan-Morales, Carlos Noe, Cervantes-Salazar, Margot, Bolaños, Jeni, Cigarroa-Mayorga, Oscar E., Martín-Martínez, Eduardo San, Medina, Fernando, Fragoso-Soriano, Rogelio Jaime, Chávez-Munguía, Bibiana, Salas-Benito, Juan Santiago, del Angel, Rosa M.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Netherlands Elsevier B.V 01.06.2019
Témata:
Aedes
Aedes albopictus
Animals
antibodies
arboviruses
C6/36 cells
cell communication
Cell Line
cross reaction
Dengue - metabolism
Dengue - virology
Dengue virus
Dengue Virus - physiology
Dynamic Light Scattering
Exosomes
Exosomes - immunology
Exosomes - metabolism
Exosomes - virology
extracellular space
Humans
Insect Proteins - chemistry
Insect Proteins - genetics
Insect Proteins - immunology
Insect Proteins - metabolism
Intraluminal vesicles (ILVs)
Microscopy, Confocal
Microscopy, Electron, Transmission
Mosquito Vectors - classification
Mosquito Vectors - genetics
Mosquito Vectors - metabolism
Mosquito Vectors - virology
Multivesicular bodies (MVBs)
Phylogeny
Tetraspanins - chemistry
Tetraspanins - genetics
Tetraspanins - immunology
Tetraspanins - metabolism
Virus Replication
virus transmission
virus-like particles
Intraluminal vesicles (ILVs)
C6/36 cells
Multivesicular bodies (MVBs)
Dengue virus
Exosomes
ISSN:0168-1702, 1872-7492, 1872-7492
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Shrnutí:•Exosomes from mosquito cells contain proteins homologs to human tetraspanins.•Exosomes from dengue virus-infected mosquito cells are larger than the ones from uninfected cells.•Exosomes from dengue virus-infected mosquito cells can infect naïve mosquito cells.•Exosomes play a role in virus dissemination. Exosomes are endocytic origin small-membrane vesicles secreted to the extracellular space by most cell types. Exosomes released from virus infected-cells can mediate the cell-to-cell communication to promote or modulate viral transmission. Dengue virus (DENV) is an arbovirus transmitted by Aedes mosquitoes bite to humans. Interestingly, the role of exosomes during the DENV infection in mammalian cells has already been described. However, little is known about exosomes derived from infected mosquito cells. Thus, the exosomes released from DENV-infected C6/36 cells were isolated, purified and analyzed using an antibody against the tetraspanin CD9 from human that showed cross-reactivity with the homologs to human CD9 found in Aedes albopictus (AalCD9). The exosomes from DENV infected cells were larger than the exosomes secreted from uninfected cells, contained virus-like particles, and they were able to infect naïve C6/36 cells, suggesting that exosomes are playing a role in virus dissemination.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0168-1702
1872-7492
1872-7492
DOI:10.1016/j.virusres.2019.03.015