The Relationship between the Hydrolysis Degree and Calcium-Binding Capacity of Whey Protein by Enzymatic Hydrolysis

To study the relationship between the hydrolysis degree and calcium-binding capacity of whey protein by enzymatic hydrolysis, the response surface method was firstly used to investigate optimized the hydrolysis conditions of whey protein with protamex and flavorzyme. The optimum process parameters f...

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Bibliographic Details
Published in:Applied Mechanics and Materials Vol. 541-542; no. Engineering and Manufacturing Technologies; pp. 214 - 219
Main Authors: Huang, Shun Li, Huang, Yi Fan, Zhao, Li Na, Wang, Shao Yun
Format: Journal Article
Language:English
Published: Zurich Trans Tech Publications Ltd 12.03.2014
Subjects:
Calcium
Chelation
Hydrolysates
Hydrolysis
Optimization
Proteins
Response surfaces
Whey
Enzymatic Hydrolysis
Whey Protein
Response Surface Method (RSM)
Calcium-Binding Ability
ISBN:9783038350552, 3038350559
ISSN:1660-9336, 1662-7482, 1662-7482
Online Access:Get full text
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Summary:To study the relationship between the hydrolysis degree and calcium-binding capacity of whey protein by enzymatic hydrolysis, the response surface method was firstly used to investigate optimized the hydrolysis conditions of whey protein with protamex and flavorzyme. The optimum process parameters for the whey protein hydrolysis were as follows: Whey protein concentration was 5.0% (w/v), the ratio of protease to whey protein was 4.0% (w/w), the mass ratios of protamex to flavorzyme (w/w) was 2:1, and the reaction temperature was 49 °C. The hydrolysate obtained after the hydrolysis of 7 h, with a hydrolysis degree of 25.92%, possessed the highest Ca-binding capacity of 27.92%. Finally, the relationship between the hydrolysis degree and calcium-binding capacity was established and whey protein hydrolysate with high calcium-binding capacity was prepared, which can provide basic theories for the following optimization of chelation of whey protein hydrolysate with calcium.
Bibliography:Selected, peer reviewed papers from the 2014 5th International Conference on Mechanical, Industrial, and Manufacturing Technologies (MIMT 2014), March 10-11, 2014, Penang, Malaysia
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ISBN:9783038350552
3038350559
ISSN:1660-9336
1662-7482
1662-7482
DOI:10.4028/www.scientific.net/AMM.541-542.214