Influence of red wine polysaccharides on cytochrome P450 enzymes and inflammatory parameters in tumor models

The soluble fraction of polysaccharides from cabernet franc red wine (SFP) previously showed antitumoral effects by modulating the immune system. The present study tested the hypothesis that the SFP can regulate CYPs in vitro in HepG2 cells and in vivo in Walker-256 tumor-bearing rats. The SFP was u...

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Vydané v:International journal of biological macromolecules Ročník 240; s. 124385
Hlavní autori: Stipp, Maria Carolina, Kulik, Juliana Danna, Corso, Claudia Rita, Galindo, Claudia Martins, Adami, Eliana Rezende, Evangelista, Alberto Gonçalves, Luciano, Fernando Bittencourt, Winnischofer, Sheila Maria Brochado, Cadena, Silvia Maria Suter Correia, Sassaki, Guilherme Lanzi, Acco, Alexandra
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Netherlands Elsevier B.V 15.06.2023
Predmet:
Acetylglucosaminidase
Animals
cancer
chemoprevention
cytochrome P-450
Cytochrome P-450 CYP1A1 - genetics
Cytochrome P-450 CYP1A1 - metabolism
Cytochrome P-450 Enzyme System - metabolism
Cytochrome P450
digestion
gene expression
immune system
Inflammation
intestines
liquids
liver
myeloperoxidase
neoplasms
nuclear magnetic resonance spectroscopy
polysaccharides
Polysaccharides - pharmacology
Rats
red wines
vincristine
Wine
INF
ALT
MTT
MPO
DMEM
ANOVA
FBS
qPCR
CYP
TLR
SFP
D2O
PBS
AST
IL-1
Cytochrome P450
s.c
i.p
Inflammation
EDTA
IL-6
rpm
PAMP
NMR
TNF-α
NAG
Veh
SEM
Vin
cancer
GAPDH
VS
ELISA
ISSN:0141-8130, 1879-0003, 1879-0003
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Shrnutí:The soluble fraction of polysaccharides from cabernet franc red wine (SFP) previously showed antitumoral effects by modulating the immune system. The present study tested the hypothesis that the SFP can regulate CYPs in vitro in HepG2 cells and in vivo in Walker-256 tumor-bearing rats. The SFP was used in the following protocols: (i) solid tumor, (ii) liquid tumor, and (iii) chemopreventive solid tumor. The SFP reduced solid tumor growth in both solid tumor protocols but did not inhibit liquid tumor development. The SFP reduced total CYP levels in the solid and liquid tumor protocols and reduced the gene expression of Cyp1a1 and Cyp2e1 in rats and CYP1A2 in HepG2 cells. An increase of N-acetylglucosaminidase activity was observed in all SFP-treated rats, and TNF-α levels increased in the solid tumor protocol in the vehicle, SFP, and vincristine (positive control) groups. The chemopreventive solid tumor protocol did not modify CYP levels in the liver or intestine or N-acetylglucosaminidase and myeloperoxidase activity in the liver. The in vitro digestion and nuclear magnetic resonance analyses suggested that SFP was minimally modified in the gastrointestinal system. In conclusion, SFP inhibited CYPs both in vivo and in vitro, likely as a result of its immunoinflammatory actions. [Display omitted] •The soluble fraction of polysaccharides from cabernet franc red wine (SFP) had antineoplastic effect against Walker-256 solid tumor.•SFP had antitumoral effect in treatment and chemoprentive protocols.•SFP did not inhibit liquid Walker-256 tumor development in rats.•SFP inhibited CYPs both in vivo and in vitro, likely as a result of its immunoinflammatory actions.•SFP was minimally modified in the in vitro digestion system.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0141-8130
1879-0003
1879-0003
DOI:10.1016/j.ijbiomac.2023.124385