Variability of the Chromatin Decondensation Ability Test on Human Sperm

A sperm nuclear decondensation ability test using 1% SDS + 6 mM EDTA was used to evaluate: a system of classification and nomenclature for the decondensation of nuclear chromatin; the progress of decondensation as a function of the duration of exposure to SDS/EDTA; the residual variance, or "sc...

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Bibliographic Details
Published in:Archives of andrology Vol. 11; no. 1; pp. 1 - 7
Main Author: Huret, J. L.
Format: Journal Article
Language:English
Published: England Informa UK Ltd 01.01.1983
Taylor & Francis
Subjects:
Chromatin - analysis
Chromatin decondensation
Edetic Acid
Human
Humans
Male
Methodology
Methods
Nucleus
Sodium Dodecyl Sulfate
Sperm
Spermatozoa - analysis
ISSN:0148-5016, 1521-0375
Online Access:Get full text
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Summary:A sperm nuclear decondensation ability test using 1% SDS + 6 mM EDTA was used to evaluate: a system of classification and nomenclature for the decondensation of nuclear chromatin; the progress of decondensation as a function of the duration of exposure to SDS/EDTA; the residual variance, or "scoring error;" the within-subject variance (N = 5); and the between-subject variance (N = 10). The process of chromatin decondensation was found to be a continuous phenomenon, but a scheme of nomenclature using four categories along with a system of data analysis using class weightings were developed. A 5-min exposure to SDS/EDTA resulted in a minimum scoring error (8.34%). The within- and between-subject variances were not significantly different from each other, but both were individually different (p < 0.001) from the residual variance.
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ISSN:0148-5016
1521-0375
DOI:10.3109/01485018308987451