A simple and easy non‐derivatization gas chromatography‐mass spectrometry method for simultaneous quantification of valproic acid, gabapentin, pregabalin, and vigabatrin in human plasma

Immunoassays are currently not available in commercial kits for the quantification of valproic acid, vigabatrin, pregabalin, and gabapentin, which also cannot suffer the limitations of interferences of substances with similar structures. Chromatography is a good alternative to immunoassay. In this s...

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Vydané v:Journal of separation science Ročník 46; číslo 2; s. e2200622 - n/a
Hlavní autori: Jin, Peng, You, Yu‐xin, Zhao, Lin‐lin, Zhao, Yan‐lin, Zheng, Xiao‐xiao, Du, Yan, Tang, Dao‐quan
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Germany Wiley Subscription Services, Inc 01.01.2023
Predmet:
Acidification
Amines - analysis
Anticonvulsants
antiepileptic drugs
Benzoic acid
Blood plasma
chemical species
Chromatography
Correlation coefficients
Cyclohexanecarboxylic Acids - analysis
Cyclohexanecarboxylic Acids - chemistry
Epilepsy
Gabapentin - analysis
gamma-Aminobutyric Acid
Gas chromatography
gas chromatography-mass spectrometry
Gas Chromatography-Mass Spectrometry - methods
human plasma
Humans
Hydrochloric acid
Immunoassay
immunoassays
Linearity
Mass spectrometry
non‐derivatization
Plasma
Pregabalin - analysis
Scientific imaging
separation
Spectroscopy
standard deviation
therapeutic drug monitoring
therapeutics
valproic acid
Valproic Acid - analysis
Vigabatrin - analysis
antiepileptic drugs
gas chromatography-mass spectrometry
non-derivatization
human plasma
therapeutic drug monitoring
ISSN:1615-9306, 1615-9314, 1615-9314
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Popis
Shrnutí:Immunoassays are currently not available in commercial kits for the quantification of valproic acid, vigabatrin, pregabalin, and gabapentin, which also cannot suffer the limitations of interferences of substances with similar structures. Chromatography is a good alternative to immunoassay. In this study, a simple and robust non‐derivatization gas chromatography‐mass spectrometry method for simultaneous determination of the above four drugs in human plasma was developed and validated for therapeutic drug monitoring purposes. This method employed benzoic acid as the internal standard with hydrochloric acid for plasma acidification and ACN for precipitate protein. The supernatant was directly injected into gas chromatography‐mass spectrometry for analysis. Good linearity was obtained with linear correlation coefficients of the four analytes of 0.9988–0.9996. Extraction recoveries of valproic acid, vigabatrin, pregabalin, and gabapentin were respectively in the ranges of 91.3%–94.5%, 90.0%–90.9%, 90.0%–92.1%, and 88.0%–92.2% with the relative standard deviation values less than 12.6%. Intra‐ and inter‐batch precision and accuracy, and stability assays were all acceptable. Taken together, the novel method developed in this study provided easy plasma pretreatment, good extraction yield, and high chromatographic resolution, which has been successfully validated through the quantification of valproic acid in the plasma of 46 patients with epilepsy.
Bibliografia:Those authors contributed to the work equally and should be regarded as co‐first authors.
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ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.202200622