Impact of conventional/non-conventional extraction methods on the untargeted phenolic profile of Moringa oleifera leaves

The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied. The phenolic composition of alcoholic (metha...

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Vydáno v:Food research international Ročník 115; s. 319 - 327
Hlavní autoři: Rocchetti, Gabriele, Blasi, Francesca, Montesano, Domenico, Ghisoni, Silvia, Marcotullio, Maria Carla, Sabatini, Stefano, Cossignani, Lina, Lucini, Luigi
Médium: Journal Article
Jazyk:angličtina
Vydáno: Canada Elsevier Ltd 01.01.2019
Témata:
Antioxidants
Antioxidants - analysis
Chromatography, High Pressure Liquid
Disaccharides - analysis
discriminant analysis
Extraction technologies
Flavonoids - analysis
Glucosides - analysis
Hydroxybenzoates - analysis
Italy
leaves
liquid chromatography
maceration
Mass Spectrometry
Metabolomics
methanol
microwave treatment
Moringa oleifera
Moringa oleifera - chemistry
multivariate analysis
Oxygen Radical Absorbance Capacity
Phenols - analysis
Plant Extracts - analysis
Plant Leaves - chemistry
Polyphenols
Polyphenols - analysis
protocatechuic acid
quercetin
Quercetin - analogs & derivatives
Quercetin - analysis
UHPLC-ESI/QTOF
ultrasonic treatment
Italy
Antioxidants
Polyphenols
Metabolomics
UHPLC-ESI/QTOF
Extraction technologies
ISSN:0963-9969, 1873-7145, 1873-7145
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Shrnutí:The impact of different extraction methods, namely maceration, homogenizer-assisted extraction, rapid solid-liquid dynamic extraction, microwave-assisted extraction and ultrasound-assisted extraction, on polyphenols of Moringa oleifera leaves was studied. The phenolic composition of alcoholic (methanol 100%) and hydroalcoholic (methanol/water 50:50, v/v) extracts was compared by using an untargeted metabolomics-based profiling approach followed by multivariate statistics. With this aim, ultra-high-pressure liquid chromatography coupled to a quadrupole-time-of-flight mass spectrometry was used to profile phenolic compounds under the different extraction conditions. Besides, the in vitro antioxidant activities of Moringa leaves were also investigated as ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity (ORAC). The metabolomic approach allowed to putatively annotate 262 phenolic compounds. In particular, glycosidic forms of quercetin (i.e., quercetin 3-O-galactoside, quercetin 3-O-glucoside, and quercetin 4’-O-glucoside) were the most represented compounds among flavonoids. Furthermore, protocatechuic acid was found to be the most abundant hydroxybenzaldheyde derivative, while the isomeric forms of hydroxybenzoic acid characterized the phenolic acids class. Overall, the extractions in methanol 100% were found to be the most effective for phenolic compounds recovering, when compared with those in methanol/water (50:50, v/v). Homogenizer-assisted extraction of M. oleifera leaves using 100% methanol allowed extracting the highest amounts of polyphenols (35.19 mg/g) and produced the highest oxygen radical absorbance capacity (536.27 μmol Trolox Equivalents/g). The supervised orthogonal projection to latent structures discriminant analysis identified phenolic acids as the phenolic class mostly affected by the different extraction technologies. These findings demonstrate that each extraction method promoted the recovery of specific phenolic subclasses with different efficiencies. [Display omitted] •Moringa leaves were subjected to conventional/non-conventional extractions.•Untargeted metabolomics allowed to identify 262 phenolic compounds.•FRAP and ORAC assays were used to determine the in vitro antioxidant potential.•Homogenizer-assisted extraction (methanol 100%) produced the highest phenol content.•Phenolic acids were the class most affected by the different extraction technologies.
Bibliografie:ObjectType-Article-1
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ISSN:0963-9969
1873-7145
1873-7145
DOI:10.1016/j.foodres.2018.11.046