Ultrafast LC-MS/MS analysis of 5-hydroxyindoleacetic acid (5-HIAA) in serum

A very quick and easy LC-MS/MS analysis method for 5-HIAA (5-hydoxyindoleacetic acid) has been developed. The method was fully validated and proved to work well in a clinical setting. Precision at the upper reference limit 123 nmol/L was 3,3% CV. Accuracy ranged from 96% at low levels (50-100 nmol/L...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation Vol. 81; no. 5; pp. 401 - 405
Main Authors: Oxelbark, Joakim, Lövenhamn, Annicka
Format: Journal Article
Language:English
Published: England Taylor & Francis 01.09.2021
Subjects:
5-Hydroxyindoleacetic acid
Chromatography, Liquid - methods
Humans
Hydroxyindoleacetic Acid - blood
Hydroxyindoleacetic Acid - chemistry
Limit of Detection
liquid chromatography
mass spectrometry
reference interval neuroendocrine tumor
Reference Values
serum
Tandem Mass Spectrometry - methods
5-Hydroxyindoleacetic acid
liquid chromatography
mass spectrometry
serum
reference interval neuroendocrine tumor
ISSN:0036-5513, 1502-7686, 1502-7686
Online Access:Get full text
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Summary:A very quick and easy LC-MS/MS analysis method for 5-HIAA (5-hydoxyindoleacetic acid) has been developed. The method was fully validated and proved to work well in a clinical setting. Precision at the upper reference limit 123 nmol/L was 3,3% CV. Accuracy ranged from 96% at low levels (50-100 nmol/L) to 99.7% at high levels (500 nmol/L). A previously reported reference interval of 35-123 nmol/L was confirmed as valid based on analysis of 40 samples from voluntary blood donors.
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SourceType-Scholarly Journals-1
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content type line 23
ISSN:0036-5513
1502-7686
1502-7686
DOI:10.1080/00365513.2021.1930141