Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel...

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Veröffentlicht in:Journal of controlled release Jg. 243; S. 160 - 171
Hauptverfasser: Yang, Zhaogang, Xie, Jing, Zhu, Jing, Kang, Chen, Chiang, Chiling, Wang, Xinmei, Wang, Xiaobing, Kuang, Tairong, Chen, Feng, Chen, Zhou, Zhang, Aili, Yu, Bo, Lee, Robert J., Teng, Lesheng, Lee, L. James
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Netherlands Elsevier B.V 10.12.2016
Schlagworte:
Animals
biosafety
Breast Neoplasms - genetics
cell communication
Cell Line, Tumor
drugs
Electroporation
Endocytosis
Endocytosis - physiology
Epithelial Cells - metabolism
epithelium
Exosome
Exosomes
extrusion
filters
gene transfer
Gene Transfer Techniques
Humans
in vivo studies
MCF-7
MCF-7 Cells
Mice
Mice, Inbred BALB C
Mice, Nude
neoplasms
Particle Size
proteomics
RNA, Small Interfering - administration & dosage
siRNA
small interfering RNA
Electroporation
Endocytosis
siRNA
Exosome
MCF-7
ISSN:0168-3659, 1873-4995
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Zusammenfassung:Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied. [Display omitted]
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0168-3659
1873-4995
DOI:10.1016/j.jconrel.2016.10.008