Necroptosis mediates the antineoplastic effects of the soluble fraction of polysaccharide from red wine in Walker-256 tumor-bearing rats

•Soluble fraction of polysaccharide extracted from cabernet franc wine has a complex mixture, with arabinogalactans, mannans and rhamnogalacturonans.•Soluble fraction of polysaccharide showed and expressive antineoplastic effects against the Walker-256 tumor in rats.•Soluble fraction of polysacchari...

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Bibliographic Details
Published in:	Carbohydrate polymers Vol. 160; pp. 123 - 133
Main Authors:	Stipp, Maria Carolina, Bezerra, Iglesias de Lacerda, Corso, Claudia Rita, dos Reis Livero, Francislaine A., Lomba, Luiz Alexandre, Caillot, Adriana Rute Cordeiro, Zampronio, Aleksander Roberto, Queiroz-Telles, José Ederaldo, Klassen, Giseli, Ramos, Edneia A.S., Sassaki, Guilherme Lanzi, Acco, Alexandra
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 15.03.2017
Subjects:	Animals antineoplastic activity Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Apoptosis buffered 10% formalin cabernet franc dimethylformamide 8 ethanol ethylenediaminetetraacetic acid (EDTA) (0.5 M, pH 8.0) eTrypan blue, 2 M trifluoroacetic acid genes Griess solution (0.1% N-1-naphthyl-tilediamine, 1% sulfanilamide in 5% H3PO4) hematoxylin and eosin (HE) immunomodulation ketamine hydrochloride lymphocytes mannans metothrexate monocytes myeloperoxidase N-acetyl-β-d-glucosamine necroptosis Neoplasms, Experimental - drug therapy neutrophils nitric oxide p-nitrofen p-nitrophenyl-N-acetyl-β-d-glucosamine paraffin pectins phosphate buffer (pH 6.5) phosphate buffered saline (PBS, 16.5 mM phosphate, 137 mM NaCl, and 2.7 mM KCl) at pH 7.4 polysaccharide Polysaccharides - chemistry Polysaccharides - pharmacology Protein Serine-Threonine Kinases - metabolism Rats Receptor-Interacting Protein Serine-Threonine Kinases - metabolism red wine red wines saline Triton X-100 0.1%, TMB 18.4 mM sodium acetate (NaOAc) TriZol Reagent tumor necrosis factor-alpha Tumor Necrosis Factor-alpha - metabolism Walker-256 tumor Wine xylazine hydrochloride xylol red wine polysaccharide phosphate buffer (pH 6.5) ALT Walker-256 tumor buffered 10% formalin paraffin MPO ketamine hydrochloride p53 MTX NF-κB saline Triton X-100 0.1%, TMB 18.4 mM necroptosis p-nitrophenyl-N-acetyl-β-d-glucosamine Gapdh cabernet franc p-nitrofen ROS hematoxylin and eosin (HE) Bcl-2 NO immunomodulation dimethylformamide 8 Mlkl Vegf mRNA phosphate buffered saline (PBS, 16.5 mM phosphate, 137 mM NaCl, and 2.7 mM KCl) at pH 7.4 ethanol N-acetyl-β-d-glucosamine ANOVA xylazine hydrochloride FADD SFP TriZol Reagent AST metothrexate NaOAc Griess solution (0.1% N-1-naphthyl-tilediamine, 1% sulfanilamide in 5% H3PO4) TMB Rip-1 Rip-3 eTrypan blue, 2 M trifluoroacetic acid TNF-α NAG DNA ethylenediaminetetraacetic acid (EDTA) (0.5 M, pH 8.0) Bax xylol sodium acetate (NaOAc) HE Griess solution (0.1% N-1-naphthyl-tilediamine, 1% sulfanilamide in 5% HPO)
ISSN:	0144-8617, 1879-1344
Online Access:	Get full text
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