Subset-Specific Expression of Toll-Like Receptors by Bovine Afferent Lymph Dendritic Cells

Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1-10 mRNA expression by using quantitative real-time PCR in h...

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Vydáno v:Frontiers in veterinary science Ročník 4; s. 44
Hlavní autoři: Werling, Dirk, Hope, Jayne C., Siddiqui, Nazneen, Widdison, Stephanie, Russell, Chris, Sopp, Paul, Coffey, Tracey J.
Médium: Journal Article
Jazyk:angličtina
Vydáno: Switzerland Frontiers Media S.A 03.04.2017
Témata:
Veterinary Science
bovine
dendritic cell subsets
afferent lymph
toll-like receptors
dendritic cell
ISSN:2297-1769, 2297-1769
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Shrnutí:Within the ruminant system, several possibilities exist to generate dendritic cells migrating out from the tissue into the regional draining lymph nodes as afferent lymph dendritic cells (ALDCs). Here, we analyzed toll-like receptor (TLR) 1-10 mRNA expression by using quantitative real-time PCR in highly purified subsets of bovine ALDC. As TLR expression may be influenced by pathogens or vaccines and their adjuvant, it is necessary to understand what TLRs are expressed in a steady-state system to elucidate specific differences and to potentially optimize targeted vaccines. In this study, we have assessed the TLR expression profiles of the four main bovine ALDC subsets [cDC1 and cDC2 (subsets 2-4)]. We demonstrate differences in TLR expression between the four subsets that may reflect the ability of these cells to respond to different pathogens or to respond to adjuvants.
Bibliografie:ObjectType-Article-1
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Edited by: Scott Napper, University of Saskatchewan, Canada
Specialty section: This article was submitted to Veterinary Infectious Diseases, a section of the journal Frontiers in Veterinary Science
Reviewed by: Kieran G. Meade, Teagasc – The Irish Agriculture and Food Development Authority, Ireland; Hans-Joachim Schuberth, University of Veterinary Medicine, Germany
Present address: Jayne C. Hope, The Roslin Institute & R(D)SVS, University of Edinburgh, Easter Bush, Midlothian, UK; Paul Sopp, The Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, UK; Tracey J. Coffey, School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington, Leicestershire, UK
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2017.00044