The Effect of Doxycycline Hyclate, Chlorhexidine Gluconate, and Minocycline Hydrochloride on Osteoblastic Proliferation and Differentiation In Vitro

Background: The purpose of this study was to determine the effect of the active substance of three types of local delivery systems, doxycycline hyclate 10% (DOXY), chlorhexidine gluconate, 2.5 mg (CHX), and minocycline hydrochloride, 1 mg (MINO), on osteoblastic cell proliferation and differentiatio...

Full description

Saved in:
Bibliographic Details
Published in:Journal of periodontology (1970) Vol. 80; no. 6; pp. 999 - 1005
Main Authors: Almazin, Salah M., Dziak, Rosemary, Andreana, Sebastiano, Ciancio, Sebastian G.
Format: Journal Article
Language:English
Published: Chicago, IL American Academy of Periodontology 01.06.2009
Subjects:
Alkaline Phosphatase - analysis
Anti-Bacterial Agents - pharmacology
Anti-Bacterial Agents - toxicity
Anti-Infective Agents, Local - pharmacology
Anti-Infective Agents, Local - toxicity
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiseptics
Biological and medical sciences
Biomarkers - analysis
Cell Adhesion - drug effects
Cell Differentiation - drug effects
Cell Nucleus - drug effects
Cell Proliferation - drug effects
Cell Shape - drug effects
Cell Survival - drug effects
Cells, Cultured
Chlorhexidine - analogs & derivatives
Chlorhexidine - pharmacology
Chlorhexidine - toxicity
Coloring Agents
Doxycycline - analogs & derivatives
Doxycycline - pharmacology
Doxycycline - toxicity
Drug Carriers
Drugs
Female
Gels
Humans
Male
Medical sciences
Microscopy, Electron, Scanning
Microspheres
Middle Aged
Minocycline - pharmacology
Minocycline - toxicity
osteoblasts
Osteoblasts - drug effects
Otorhinolaryngology. Stomatology
periodontium
Pharmacology. Drug treatments
Pseudopodia - drug effects
Tetrazolium Salts
Thiazoles
Trypan Blue
Drugs
Dentistry
In vitro
Antibiotic
Periodontal ligament
periodontium
Antiseptic
Doxycycline
Hyclate
Minocycline
Osteoblast
Chlorhexidine
osteoblasts
Antibacterial agent
Differentiation
Disinfecting agent
ISSN:0022-3492, 1943-3670
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Background: The purpose of this study was to determine the effect of the active substance of three types of local delivery systems, doxycycline hyclate 10% (DOXY), chlorhexidine gluconate, 2.5 mg (CHX), and minocycline hydrochloride, 1 mg (MINO), on osteoblastic cell proliferation and differentiation. Methods: There were four groups: control osteoblastic cells (OB) alone, OB + DOXY, OB + CHX, and OB + MINO. Trypan blue and MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] assays were used to test osteoblastic cell viability. Cell differentiation was tested by measuring alkaline phosphatase levels. Osteoblast morphology was investigated by light and scanning electron microscopy. Results: At a concentration of 0.5 mg/ml, the Trypan blue test showed that DOXY, MINO, and CHX had significant toxicity effects on osteoblast cells compared to the control group, with a mean cell viability of 84%, 74%, and 51%, respectively (P <0.05). The MTT test showed that the control and DOXY groups were statistically significantly different (P <0.05) compared to CHX and MINO groups. The DOXY group showed a significantly higher alkaline phosphatase activity (∼56%) than the control and MINO groups, and it was nearly 178% higher than the CHX group (P <0.05). The morphology of the osteoblasts seemed to be slightly altered when they were incubated with DOXY; however, with MINO, they appeared rounded with minimal attachment. In the CHX group, the osteoblasts assumed a shape of a very thin filopodia with a volcano‐like nucleus. Conclusions: At a concentration of 0.5 mg/ml, CHX and, to a lesser extent, MINO had a cytotoxic effect on osteoblast proliferation in vitro. However, DOXY seemed to enhance maturation and differentiation rather than proliferation. In addition to DOXY's beneficial effect as an adjunctive therapy to mechanical debridement in the treatment of periodontal disease, it may have an effect on periodontal regeneration.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0022-3492
1943-3670
DOI:10.1902/jop.2009.080574