Standardized method for mechanistic modeling of multimodal anion exchange chromatography in flow through operation

•Hydrophobic interaction chromatography (HIC) isotherms employed for anionic multimodal modeling.•Model parameters analytically determined using negative linear gradient elution (LGE).•Standardized modeling workflow validated for four different antibody formats. Multimodal chromatography offers an i...

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Vydané v:	Journal of Chromatography A Ročník 1690; s. 463789
Hlavní autori:	Hess, Rudger, Yun, Doil, Saleh, David, Briskot, Till, Grosch, Jan-Hendrik, Wang, Gang, Schwab, Thomas, Hubbuch, Jürgen
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Netherlands Elsevier B.V 08.02.2023
Predmet:	adsorption Analytical parameter determination anion exchange chromatography antibodies Antibodies, Monoclonal - chemistry biopharmaceuticals Chromatography, Ion Exchange - methods Computer Simulation hydrophobicity immunoglobulin G Mixed-mode gradient elution Model calibration and validation parameter uncertainty Parameter uncertainty assessment prediction Sodium Chloride sorption isotherms Therapeutic antibody Analytical parameter determination Parameter uncertainty assessment Therapeutic antibody Mixed-mode gradient elution Model calibration and validation
ISSN:	0021-9673, 1873-3778, 1873-3778
On-line prístup:	Získať plný text
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