Genome‐wide comparative analysis between Cranoglanis bouderius and Pangasianodon hypophthalmus : Reveal the genes related to resistance to low‐temperature stress

Previous studies have indicated that Cranoglanis bouderius and Pangasianodon hypophthalmus clustered into a sister group. However, there was a significant difference in their minimum tolerated temperatures. To reveal the temperature adaptation‐related genes, a genome‐wide comparative analysis was pe...

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Veröffentlicht in:Journal of the World Aquaculture Society Jg. 54; H. 5; S. 1367 - 1385
Hauptverfasser: Xie, Shaolin, Wang, Dongjie, Hu, Yun, Wang, Qiujie, Zuo, Zhiheng, Ye, Bin, Lu, Liang, Zhou, Aiguo, Zou, Jixing
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Baton Rouge John Wiley & Sons, Inc 01.10.2023
Wiley
Schlagworte:
Analysis
aquaculture
C. bouderius
Calcium
Calcium (mitochondrial)
Calcium homeostasis
Calcium ions
Calcium transport
Cell fusion
Cells
Chromosomes
cold stress
Comparative analysis
Cranoglanis bouderius
gene ontology
Genes
genome
genome assembly
Genomes
genome‐wide comparative analysis
Homeostasis
Ion transport
low‐temperature stress
mitochondria
mitochondrial fusion
Pangasianodon hypophthalmus
Temperature
North America
Vietnam
China
ISSN:0893-8849, 1749-7345
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Zusammenfassung:Previous studies have indicated that Cranoglanis bouderius and Pangasianodon hypophthalmus clustered into a sister group. However, there was a significant difference in their minimum tolerated temperatures. To reveal the temperature adaptation‐related genes, a genome‐wide comparative analysis was performed. First, a chromosome‐level draft genome of C. bouderius was constructed in this study. The genome assembly was 999.18 Mb in size with a contig N50 of 20.47 Mb. Then, an additional 118.98 Gb of Hi‐C data was applied to assemble contigs into scaffolds and 910.59 Mb was anchored and orientated onto 38 chromosomes of C. bouderius . A total of 24,165 protein‐coding genes were predicted from the genome with 22,920 (94.84%) genes functionally annotated. Genome‐wide comparative analysis revealed that the genes related to resistance to low‐temperature stress were mainly enriched into the gene ontology (GO) terms associated with mitochondrial fusion and calcium ion transport. Further, the low‐temperature stress test on the C. bouderius and P. hypophthalmus also revealed that the C. bouderius can better control the homeostasis of calcium ions in cells than P. hypophthalmus , and then better maintain the dynamic changes of mitochondrial fusion and fission in cells, thereby resisting cell damage caused by low‐temperature stress.
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ISSN:0893-8849
1749-7345
DOI:10.1111/jwas.12955