LC-MS/MS analysis of 11-nor-9-carboxy-hexahydrocannabinol (HHC-COOH) and 11-hydroxy-hexahydrocannabinol (HHC-OH) for verification of hexahydrocannabinol (HHC) intake

Natural and semi-synthetic cannabinoid analogs are getting increasing media attention for their recreative use as an alternative to traditional cannabis, in Sweden as well as internationally. To investigate an increasing number of urine samples incoming to our clinical laboratory that were screening...

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Vydáno v:Scandinavian journal of clinical and laboratory investigation Ročník 84; číslo 2; s. 109 - 114
Hlavní autoři: Pettersson-Pablo, Paul, Oxelbark, Joakim
Médium: Journal Article
Jazyk:angličtina
Vydáno: England 01.04.2024
Témata:
cannabinoids
Cannabis
Dronabinol - analogs & derivatives
Dronabinol - urine
Gas Chromatography-Mass Spectrometry - methods
Humans
hydrolysis
immunoassay
Liquid Chromatography-Mass Spectrometry
narcotics
Substance Abuse Detection - methods
Tandem Mass Spectrometry - methods
urine
Cannabis
narcotics
urine
cannabinoids
liquid Chromatography-Mass spectrometry
hydrolysis
immunoassay
ISSN:0036-5513, 1502-7686, 1502-7686
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Shrnutí:Natural and semi-synthetic cannabinoid analogs are getting increasing media attention for their recreative use as an alternative to traditional cannabis, in Sweden as well as internationally. To investigate an increasing number of urine samples incoming to our clinical laboratory that were screening positive, using a CEDIA THC-COOH immunoassay from ThermoFisher Scientific, but then testing negative using GC-MS based verification analysis, we developed an LC-MS/MS-method for verification of hexahydrocannabinol (HHC) and Δ8-tetrahydrocannabinol. Assessment of HHC intake was based on identification of the following four metabolites: 11-nor-9(R)-carboxy-hexahydrocannabinol (R-HHC-COOH), 11-nor-9(S)-carboxy-hexahydrocannabinol (S-HHC-COOH), 11-hydroxy-9(R)-hexahydrocannabinol (R-HHC-OH) and 11-hydroxy-9(S)-hexahydrocannabinol (S-HHC-OH). Out of 46 urine samples analysed in this study, 44 showed presence of HHC-metabolites, which indicate HHC as the main explanation for an increased number of negative verifications for THC-COOH. In these samples, the HHC-OH metabolites occurred at a higher concentration than R-HHC-COOH while S-HHC-COOH was only detected in few samples at low concentrations. R-HHC-COOH and S-HHC-COOH can easily be added to a pre-existing verification method for THC-COOH, and still show acceptable results, while HHC-OH requires an enzyme capable of hydrolysing the ether glucuronide bond.
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ISSN:0036-5513
1502-7686
1502-7686
DOI:10.1080/00365513.2024.2333023