Simultaneous separation and quantitation of the major bovine whey proteins including proteose peptone and caseinomacropeptide by reversed-phase high-performance liquid chromatography on polystyrene–divinylbenzene

A precise, sensitive and reliable RP-HPLC method was developed to enable not only unequivocal determination of α-lactalbumin and β-lactoglobulin in bovine whey samples, but also simultaneous measurement of proteose peptone, caseinomacropeptide, bovine serum albumin and immunoglobulin G. The optimise...

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Bibliographic Details
Published in:	Journal of Chromatography A Vol. 878; no. 2; pp. 183 - 196
Main Authors:	Elgar, David F., Norris, Carmen S., Ayers, John S., Pritchard, Mark, Otter, Don E., Palmano, Kate P.
Format:	Journal Article
Language:	English
Published:	Netherlands Elsevier B.V 12.05.2000
Subjects:	Amino Acids Amino Acids - analysis analysis Animals Caseinomacropeptide Caseins Caseins - analysis Caseins - isolation & purification Cattle chemistry Chromatography, Affinity Chromatography, High Pressure Liquid Chromatography, High Pressure Liquid - methods Chromatography, Ion Exchange high performance liquid chromatography isolation & purification methods Milk Proteins Milk Proteins - analysis Milk Proteins - isolation & purification Peptide Fragments Peptide Fragments - analysis Peptide Fragments - isolation & purification Peptones Peptones - analysis Peptones - isolation & purification Polystyrenes Polystyrenes - chemistry Proteins Proteose peptone Reproducibility of Results Whey Proteins Proteins Caseinomacropeptide Proteose peptone Whey Food analysis
ISSN:	0021-9673
Online Access:	Get full text
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