Quantification of the sensitivity increase of a micro-high-performance liquid chromatography–electrospray ionization mass spectrometry system with decreasing column diameter

This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compar...

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Veröffentlicht in:Journal of Chromatography A Jg. 976; H. 1; S. 11 - 18
Hauptverfasser: Legido-Quigley, C, Smith, N.W, Mallet, D
Format: Journal Article Tagungsbericht
Sprache:Englisch
Veröffentlicht: Amsterdam Elsevier B.V 08.11.2002
Elsevier
Schlagworte:
Analysis
Analytical chemistry
Biological and medical sciences
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography, High Pressure Liquid - methods
Exact sciences and technology
General pharmacology
Medical sciences
Other chromatographic methods
Pharmacology. Drug treatments
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods
Spectrophotometry, Ultraviolet
Sensitivity
Liquid chromatography
Column diameter
Capillary column
Corticosteroid
Coupled method
Bronchodilator
Antiinflammatory agent
Salmeterol
HPLC chromatography
β-Adrenergic receptor agonist
Fluticasone
Antiasthma agent
Experimental study
Electrospray
Microbore column
Reversed phase chromatography
Mass spectrometry
Diameter
ISSN:0021-9673
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Beschreibung
Zusammenfassung:This study details the sensitivity achieved with capillary columns when used with a micro-HPLC–electrospray ionization MS system. It is comprised of two sections, the first is the comparative study of three columns, one of narrow-bore diameter and two of capillary diameter. The second section compares three columns of decreasing diameter in the capillary scale. All the experiments achieved enhanced sensitivity using capillary columns. The increase in the experimental MS response ranged from −20% to +20% compared to the UV experimental response when decreasing the internal diameter of the columns used. When comparing the experimental MS response to the maximum theoretical UV response achievable, the increase in response ranged from 40 to 50%.
Bibliographie:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0021-9673
DOI:10.1016/S0021-9673(02)01156-1