Guidelines and considerations for conducting experiments using tissue microarrays

Tissue microarrays (TMAs) represent a powerful method for undertaking large‐scale tissue‐based biomarker studies. While TMAs offer several advantages, there are a number of issues specific to their use which need to be considered when employing this method. Given the investment in TMA‐based research...

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Veröffentlicht in:Histopathology Jg. 62; H. 6; S. 827 - 839
Hauptverfasser: Ilyas, Mohammad, Grabsch, Heike, Ellis, Ian O, Womack, Chris, Brown, Robert, Berney, Dan, Fennell, Dean, Salto-Tellez, Manuel, Jenkins, Martin, Landberg, Goran, Byers, Richard, Treanor, Darren, Harrison, David, Green, Andrew R, Ball, Graham, Hamilton, Peter
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England Blackwell Publishing Ltd 01.05.2013
Wiley Subscription Services, Inc
Schlagworte:
Academies and Institutes
Biomarkers
Biomarkers - metabolism
data interpretation
Data Interpretation, Statistical
experimental design
Experiments
guidance
Humans
Immunohistochemistry
Medical research
Quality Control
Studies
Tissue Array Analysis - methods
Tissue Array Analysis - standards
Tissue Array Analysis - statistics & numerical data
tissue microarray
United Kingdom
ISSN:0309-0167, 1365-2559, 1365-2559
Online-Zugang:Volltext
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Zusammenfassung:Tissue microarrays (TMAs) represent a powerful method for undertaking large‐scale tissue‐based biomarker studies. While TMAs offer several advantages, there are a number of issues specific to their use which need to be considered when employing this method. Given the investment in TMA‐based research, guidance on design and execution of experiments will be of benefit and should help researchers new to TMA‐based studies to avoid known pitfalls. Furthermore, a consensus on quality standards for TMA‐based experiments should improve the robustness and reproducibility of studies, thereby increasing the likelihood of identifying clinically useful biomarkers. In order to address these issues, the National Cancer Research Institute Biomarker and Imaging Clinical Studies Group organized a 1‐day TMA workshop held in Nottingham in May 2012. The document herein summarizes the conclusions from the workshop. It includes guidance and considerations on all aspects of TMA‐based research, including the pre‐analytical stages of experimental design, the analytical stages of data acquisition, and the postanalytical stages of data analysis. A checklist is presented which can be used both for planning a TMA experiment and interpreting the results of such an experiment. For studies of cancer biomarkers, this checklist could be used as a supplement to the REMARK guidelines.
Bibliographie:Pathological Society of Great Britain and Ireland
GE Systems
ArticleID:HIS12118
Philips
The National Cancer Research Institute
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ObjectType-Article-1
SourceType-Scholarly Journals-1
content type line 14
ObjectType-Article-2
ObjectType-Instructional Material/Guideline-1
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ISSN:0309-0167
1365-2559
1365-2559
DOI:10.1111/his.12118