Detection of Maedi-Visna virus antibodies using a single fusion transmembrane-core p25 recombinant protein ELISA and a modified receiver-operating characteristic analysis to determine cut-off values

The core p25 and transmembrane (TM) genes of Maedi-Visna virus (MVV) were cloned individually into the pGEX-2T expression vector. Both proteins were expressed as a combined fusion protein in frame with glutathione S-transferase (GST). The purified recombinant antigens (GST-TM and GST-TM-p25) were us...

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Bibliographic Details
Published in:Journal of virological methods Vol. 63; no. 1; pp. 47 - 56
Main Authors: Boshoff, C.H., Dungu, B., Williams, R., Vorster, J., Conradie, J.D., Verwoerd, D.W., York, D.F.
Format: Journal Article
Language:English
Published: London Elsevier B.V 1997
Amsterdam Elsevier
New York, NY
Subjects:
Animal viral diseases
Animals
Antibodies, Viral - blood
Antibodies, Viral - immunology
Antigens, Viral - genetics
Antigens, Viral - immunology
Biological and medical sciences
Cloning, Molecular
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Escherichia coli
Gene Products, gag - genetics
Gene Products, gag - immunology
Infectious diseases
Maedi-Visna virus diagnosis
Medical sciences
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - immunology
Sheep
Two-graph receiver operating characteristic
Viral diseases
Visna - blood
Visna - immunology
Visna - virology
Visna-maedi virus - immunology
Visna-maedi virus - isolation & purification
Maedi-Visna virus diagnosis
Two-graph receiver operating characteristic
ELISA
Transmembrane protein
Antibody
Retroviridae
Visna virus
Infection
Virus
Vertebrata
Mammalia
Viral disease
Animal
Serological method
Lentivirinae
Sheep
Artiodactyla
Diagnosis
Recombinant protein
Veterinary
Detection
Maedi
ELISA assay
Ungulata
Visna
Maedi virus
ISSN:0166-0934, 1879-0984
Online Access:Get full text
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Summary:The core p25 and transmembrane (TM) genes of Maedi-Visna virus (MVV) were cloned individually into the pGEX-2T expression vector. Both proteins were expressed as a combined fusion protein in frame with glutathione S-transferase (GST). The purified recombinant antigens (GST-TM and GST-TM-p25) were used to develop a MVV ELISA. A preliminary assessment of the diagnostic potential of the recombinant antigens (GST-TM and GST-TM-p25) was made by testing the antigens against 46 seropositive and 46 seronegative sheep and comparing the results with a commercial p25 ELISA kit. A two-graph receiver operating characteristic (TG-ROC) analysis program was used to interpret the data. The GST-TM-p25 ELISA was more sensitive than the commercial assay which is based on the p25 antigen alone and more specific than the GST-TM ELISA.
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ISSN:0166-0934
1879-0984
DOI:10.1016/S0166-0934(96)02114-3