Nac1 facilitates pluripotency gene activation for establishing somatic cell reprogramming

Transcription factors play a central role in pluripotency transcription circuitry for establishing pluripotent reprogramming. Master transcription factors Oct4, Nanog, and Sox2 are known to form the core of the pluripotency transcription network. Other transcription factors also play critical roles...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications Vol. 518; no. 2; pp. 253 - 258
Main Authors: Choi, Hwan, Park, Hyeok Ju, Kim, Hongwon, Kim, Junyeop, Lee, Yong Kyu, Kim, Jongpil
Format: Journal Article
Language:English
Published: United States Elsevier Inc 15.10.2019
Subjects:
Embryonic stem cell
gene activation
gene regulatory networks
genes
Induced pluripotent stem cell
Nucleus accumbens-1
Pluripotency
somatic cells
transcription (genetics)
Induced pluripotent stem cell
Embryonic stem cell
Nucleus accumbens-1
Pluripotency
ISSN:0006-291X, 1090-2104, 1090-2104
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transcription factors play a central role in pluripotency transcription circuitry for establishing pluripotent reprogramming. Master transcription factors Oct4, Nanog, and Sox2 are known to form the core of the pluripotency transcription network. Other transcription factors also play critical roles for further refining the core circuitry for pluripotency in induced pluripotent stem (iPS) cells. Here, we reported that Nac1 interacted with the master pluripotent factors Oct4 and Nanog co-occupies gene promoters bound by these transcriptional factors for establishing pluripotency. Moreover, this interaction coordinates gene expression with H3K4me3 in the somatic cell reprogramming. Knockdown of Nac1 suppressed somatic cell reprogramming, whereas overexpression of Nac1 resulted in enhanced efficiency of induced pluripotent cell generation. Altogether, these results reveal the genome wide role for Nac1 in the contribution to the pluripotency circuitry and the regulation of the establishing pluripotent state. •Nac1 co-occupies gene promoters bound by Oct4 and Nanog for establishing pluripotency.•Nac1 coordinates gene expression with H3K4me3 in the somatic cell reprogramming.•Nac1 contribute to the pluripotency circuitry and the establishing pluripotent state.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2019.08.043