Renal plasticity in response to feeding in the Burmese python, Python molurus bivittatus

Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that n...

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Bibliographic Details
Published in:Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 188; pp. 120 - 126
Main Authors: Esbaugh, A.J., Secor, S.M., Grosell, M.
Format: Journal Article
Language:English
Published: United States Elsevier Inc 01.10.2015
Subjects:
absorption
Acid–base balance
Alkaline tide
Amino Acid Sequence
Animals
bicarbonates
Bicarbonates - blood
body weight
Boidae - genetics
Boidae - physiology
CA IV
carbon dioxide
Carbon Dioxide - blood
carbon dioxide enrichment
carbonate dehydratase
Carbonic anhydrase
Carbonic Anhydrases - classification
Carbonic Anhydrases - genetics
Carbonic Anhydrases - metabolism
Eating - physiology
Feeding Behavior - physiology
Female
Gene Expression
gene expression regulation
H-transporting ATP synthase
Hydrogen-Ion Concentration
Isoenzymes - classification
Isoenzymes - genetics
Isoenzymes - metabolism
Kidney
Kidney - metabolism
Kidney - physiology
kidneys
Male
Phylogeny
predators
protons
Python molurus
Rats
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
sodium
Sodium-Bicarbonate Symporters - genetics
Sodium-Bicarbonate Symporters - metabolism
Sodium-Hydrogen Exchangers - genetics
Sodium-Hydrogen Exchangers - metabolism
Specific dynamic action
Time Factors
V-type ATPase
Vacuolar Proton-Translocating ATPases - genetics
Vacuolar Proton-Translocating ATPases - metabolism
V-type ATPase
CA IV
Carbonic anhydrase
Alkaline tide
Acid–base balance
Specific dynamic action
Kidney
ISSN:1095-6433, 1531-4332, 1531-4332
Online Access:Get full text
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Summary:Burmese pythons are sit-and-wait predators that are well adapted to go long periods without food, yet subsequently consume and digest single meals that can exceed their body weight. These large feeding events result in a dramatic alkaline tide that is compensated by a hypoventilatory response that normalizes plasma pH; however, little is known regarding how plasma HCO3− is lowered in the days post-feeding. The current study demonstrated that Burmese pythons contain the cellular machinery for renal acid–base compensation and actively remodel the kidney to limit HCO3− reabsorption in the post-feeding period. After being fed a 25% body weight meal plasma total CO2 was elevated by 1.5-fold after 1day, but returned to control concentrations by 4days post-feeding (dpf). Gene expression analysis was used to verify the presence of carbonic anhydrase (CA) II, IV and XIII, Na+ H+ exchanger 3 (NHE3), the Na+ HCO3− co-transporter (NBC) and V-type ATPase. CA IV expression was significantly down-regulated at 3dpf versus fasted controls. This was supported by activity analysis that showed a significant decrease in the amount of GPI-linked CA activity in isolated kidney membranes at 3dpf versus fasted controls. In addition, V-type ATPase activity was significantly up-regulated at 3dpf; no change in gene expression was observed. Both CA II and NHE3 expression was up-regulated at 3dpf, which may be related to post-prandial ion balance. These results suggest that Burmese pythons actively remodel their kidney after feeding, which would in part benefit renal HCO3− clearance.
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ISSN:1095-6433
1531-4332
1531-4332
DOI:10.1016/j.cbpa.2015.06.029