Expression of CXCR4, the receptor for stromal cell‐derived factor‐1 on fetal and adult human lymphohematopoietic progenitors

Stromal cell‐derived factor‐1 (SDF‐1) is a CXC chemokine produced by stromal cells that acts as a chemoattractant for human CD34+ progenitor cells. We investigated the expression of CXCR4, the receptor for SDF‐1, on CD34+ cells from different hematopoietic sites and developmental stages. CXCR4 was d...

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Vydané v:European journal of immunology Ročník 29; číslo 6; s. 1823 - 1831
Hlavní autori: Aiuti, Alessandro, Tavian, Manuela, Cipponi, Arcadi, Ficara, Francesca, Zappone, Elisabetta, Hoxie, James, Peault, Bruno, Bordignon, Claudio
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Weinheim WILEY‐VCH Verlag GmbH 01.06.1999
Predmet:
B lymphocyte
CD34+ cell
Chemokine
Migration
Stromal cell‐derived factor‐1
ISSN:0014-2980, 1521-4141
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Shrnutí:Stromal cell‐derived factor‐1 (SDF‐1) is a CXC chemokine produced by stromal cells that acts as a chemoattractant for human CD34+ progenitor cells. We investigated the expression of CXCR4, the receptor for SDF‐1, on CD34+ cells from different hematopoietic sites and developmental stages. CXCR4 was detected by flow cytometry on 37 % of fetal bone marrow (BM) [gestation weeks (gw) 14 – 23] and 40 % of adult BM CD34+ cells. Interestingly, in fetal liver CD34+ cells, CXCR4 was expressed at lower levels at later stages (9 %, gw 20 – 23) compared to early stages of development (39 %, gw 7.5 – 18), suggesting a development‐related change in the migratory capacity of progenitors. CXCR4 was detected at similar levels on both phenotypically primitive and committed progenitors from fetal and adult sites. However, B cell lineage progenitor and precursor cells expressed CXCR4 at the highest density (80 % of BM CD34+/CD10+ pro‐B cells are CXCR4+). CXCR4 was also expressed in the fetal thymus in early T cell precursors and found to be down‐regulated during T cell maturation. Finally, we found that stem cell factor, alone or in combination with other cytokines, can up‐modulate CXCR4 expression on CD34+ cells by three‐ to fourfold. In conclusion, our results suggest that CXCR4 may play an important role in the local and systemic trafficking of human CD34+ cells as well as in human B lymphopoiesis and that its expression can be modulated by cytokines.
ISSN:0014-2980
1521-4141
DOI:10.1002/(SICI)1521-4141(199906)29:06<1823::AID-IMMU1823>3.0.CO;2-B