Assessment of Corneal Endothelial Barrier Function Based on “Y-Junctions”: A Finite Element Analysis

Corneal endothelial cell dysfunction is a major contributor to corneal edema, opacity, and, in severe cases, corneal blindness. Currently, no direct and reliable clinical indicator is available for evaluating the function of corneal endothelial cells. This study aimed to identify new noninvasive ind...

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Veröffentlicht in:Investigative ophthalmology & visual science Jg. 66; H. 5; S. 33
Hauptverfasser: Li, Dongfang, Duan, Haoyun, Wang, Xinhang, Lin, Zhan, Dai, Kun, Hu, Xiangyue, Zhao, Xintian, Zhou, Qingjun, Li, Zongyi, Xie, Lixin
Format: Journal Article
Sprache:Englisch
Veröffentlicht: United States The Association for Research in Vision and Ophthalmology 01.05.2025
Schlagworte:
Animals
Cornea
Corneal Diseases - physiopathology
Disease Models, Animal
Endothelium, Corneal - metabolism
Endothelium, Corneal - physiology
Endothelium, Corneal - ultrastructure
Finite Element Analysis
Humans
Intercellular Junctions - physiology
Intercellular Junctions - ultrastructure
Intraocular Pressure - physiology
Male
Mice
Mice, Inbred C57BL
Microscopy, Electron, Scanning
Rabbits
ISSN:1552-5783, 0146-0404, 1552-5783
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Zusammenfassung:Corneal endothelial cell dysfunction is a major contributor to corneal edema, opacity, and, in severe cases, corneal blindness. Currently, no direct and reliable clinical indicator is available for evaluating the function of corneal endothelial cells. This study aimed to identify new noninvasive indicators for the clinical assessment of corneal endothelial barrier function. This study established a finite element simulation model of a monolayer full-corneal endothelium to screen for sensitive indicators that reflect the barrier function of the corneal endothelium. Scanning electron microscopy (SEM) was used to observe the morphology of endothelial junctions, and immunofluorescence was used to examine the expression of fluorescent particles. The "Y-junctions" area was identified as the parameter most sensitive to changes in intraocular pressure when considering the different analytical indices obtained from the finite element model. SEM of Corneal endothelial dysfunction models in rabbits and mice further confirmed a substantial increase in the "Y-junctions" relative to control groups. Additionally, functional in vitro experiments provided further evidence of a positive relationship between larger "Y-junctions" and enhanced permeability to fluorescent particles. Finally, clinical analysis of measurements related to "Y-junctions" in patients suffering from various corneal endothelial disorders consistently revealed that these junctions were significantly larger compared to those observed in healthy control subjects. The "Y-junctions" area serves as a potentially sensitive indicator for assessing endothelial barrier integrity. Consistent observation of alterations in this area may facilitate the early identification of dysfunction in circulating endothelial cells.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1552-5783
0146-0404
1552-5783
DOI:10.1167/iovs.66.5.33