Exploration of the immune response of grass carp (Ctenopharyngodon idellus) erythrocytes during bacterial infection

In teleost blood, red blood cells (RBCs) are the most common type of cell, and they differ from mammalian RBCs in having a nucleus and other organelles. As nucleated cells, teleost RBCs contribute to the immune response against pathogens, but their antibacterial mechanism remains unclear. Here, we u...

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Vydané v:Fish & shellfish immunology Ročník 149; s. 109526
Hlavní autori: Yang, Shiyi, Bai, Yanhan, Tao, Junjie, Tu, Chengming, Chen, Bing, Huang, Xiaoman, Zhang, Linpeng, Liu, Lihan, Li, Lin, Qin, Zhendong
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: England Elsevier Ltd 01.06.2024
Predmet:
Aeromonas hydrophila
Aeromonas hydrophila - physiology
Animals
Antioxidant system
Apoptosis
bacterial infections
Carps - genetics
Carps - immunology
catalase
Ctenopharyngodon idella
erythrocytes
Erythrocytes - immunology
Escherichia coli
Escherichia coli - immunology
Escherichia coli - physiology
Escherichia coli Infections - immunology
Escherichia coli Infections - veterinary
fish
Fish Diseases - immunology
Fish Proteins - genetics
Fish Proteins - immunology
flow cytometry
GcRBCs
gene expression
gene expression regulation
genome
glutathione peroxidase
Gram-Negative Bacterial Infections - immunology
Gram-Negative Bacterial Infections - veterinary
immune response
Immunity, Innate - genetics
Inflammation
interleukin-12
interleukin-17
interleukin-4
interleukin-6
interleukin-8
mammals
organelles
reactive oxygen species
ROS
sequence analysis
shellfish
Staphylococcal Infections - immunology
Staphylococcal Infections - veterinary
Staphylococcus aureus
Staphylococcus aureus - immunology
Staphylococcus aureus - physiology
superoxide dismutase
Transcriptome - immunology
transcriptomics
tumor necrosis factors
Antioxidant system
Inflammation
GcRBCs
ROS
Apoptosis
ISSN:1050-4648, 1095-9947, 1095-9947
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Shrnutí:In teleost blood, red blood cells (RBCs) are the most common type of cell, and they differ from mammalian RBCs in having a nucleus and other organelles. As nucleated cells, teleost RBCs contribute to the immune response against pathogens, but their antibacterial mechanism remains unclear. Here, we utilized RNA-Seq to analyze gene expression patterns of grass carp (Ctenopharyngodon idellus) RBCs (GcRBCs) stimulated by Aeromonas hydrophila, Escherichia coli, and Staphylococcus aureus. Our transcriptomic data showed that bacterial stimulation generated many differentially expressed genes (DEGs). Furthermore, several inflammatory pathways responded to bacterial activation, and the TLR, IL-17, and tumor necrosis factor (TNF) signaling pathways were significantly activated based on Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Furthermore, the findings of qRT-PCR showed markedly elevated expression of various cytokines, including IL-1β, IL4, IL6, IL8, IL12, and TNFα, in GcRBCs after incubation with bacteria. Reactive oxygen species (ROS) production in GcRBCs was markedly increased after the cells were stimulated with the three bacteria, and the expression of superoxide dismutase, glutathione peroxidase, and antioxidant enzymes, including catalase, was altered. Flow cytometry analysis showed that the apoptosis rate of GcRBCs was enhanced after stimulation with the three bacteria for different times. In summary, our findings reveal that bacterial stimulation activates the immune response of GcRBCs by regulating ROS release, cytokine expression, and the antioxidant system, leading to apoptosis of GcRBCs. •The stimulation of bacteria activated the immune response of erythrocytes.•The stimulation of bacteria activated the antioxidant enzymes system of erythrocytes.•The incubation of bacteria caused apoptosis of erythrocytes.
Bibliografia:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1050-4648
1095-9947
1095-9947
DOI:10.1016/j.fsi.2024.109526