Protective effects of Urtica dioica L. on experimental testicular ischaemia reperfusion injury in rats

Summary In this study, it was aimed to examine the effects of Urtica dioica L. (UD) that has antioxidant feature in the experimental testicular I/R model in rats in terms of anti‐apoptotic and antioxidative effects. In our study, 24 male rats were divided into three groups: control group, I/R group...

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Published in:	Andrologia Vol. 49; no. 4; pp. e12636 - n/a
Main Authors:	Aktas, C., Erboga, M., Fidanol Erboga, Z., Bozdemir Donmez, Y., Topcu, B., Gurel, A.
Format:	Journal Article
Language:	English
Published:	Germany John Wiley & Sons, Inc 01.05.2017
Subjects:	Animals Antioxidants - therapeutic use Apoptosis - drug effects Catalase - analysis Cell Proliferation Disease Models, Animal Glutathione Peroxidase - analysis Immunohistochemistry In Situ Nick-End Labeling ischaemia and reperfusion Lipid Peroxidation - drug effects Male Malondialdehyde - analysis Oxidative Stress - drug effects Plant Extracts - therapeutic use Proliferating Cell Nuclear Antigen - analysis Rats Reperfusion Injury - drug therapy Seeds Seminiferous Tubules - anatomy & histology Superoxide Dismutase - analysis Testis - anatomy & histology Testis - drug effects Testis - metabolism testis‐apoptosis‐oxidative stress Urtica dioica Urtica dioica - chemistry Urtica dioica L Urtica dioica L testis-apoptosis-oxidative stress ischaemia and reperfusion
ISSN:	0303-4569, 1439-0272, 1439-0272
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Summary:	Summary In this study, it was aimed to examine the effects of Urtica dioica L. (UD) that has antioxidant feature in the experimental testicular I/R model in rats in terms of anti‐apoptotic and antioxidative effects. In our study, 24 male rats were divided into three groups: control group, I/R group and I/R + UD (2 mg kg−1) group. Seminiferous tubule calibre measurement, Johnson score, haematoxylin–eosin staining, proliferative cell nucleus antigen (PCNA) immunohistochemical staining and TUNEL as histopathological have been conducted. The structural deterioration in the testicular on I/R group has reduced after the treatment of UD. Our data indicate a significant reduction in the activity of in situ identification of apoptosis using terminal dUTP nick end labelling (TUNEL), and there was a rise in the expression of proliferating cell nuclear antigen (PCNA) in testis tissues of UD‐treated rats in the I/R group. The I/R + UD group showed a decrease in malondialdehyde levels and an increase in the activities of superoxide dismutase, catalase and glutathione peroxidase in comparison with the I/R group. It could be concluded that protective effects of UD on the I/R testicles are via reduction of histological damage, apoptosis, oxidative stress and lipid peroxidation.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0303-4569 1439-0272 1439-0272
DOI:	10.1111/and.12636