Geographical distribution, genetic studies and vector transmission of alfalfa enamovirus-1 infecting alfalfa crop in Argentina

Alfalfa ( Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevale...

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Bibliographic Details
Published in:Virus genes Vol. 56; no. 5; pp. 662 - 667
Main Authors: Trucco, Verónica, Cabrera Mederos, Dariel, Lenardon, Sergio, Giolitti, Fabian
Format: Journal Article
Language:English
Published: New York Springer US 01.10.2020
Springer Nature B.V
Subjects:
alfalfa
Amino acid sequence
amino acids
Aphis craccivora
Argentina
Biomedical and Life Sciences
Biomedicine
CP gene
Enamovirus
genes
Genetic analysis
Genetic diversity
genetic variation
Geographical distribution
insect vectors
Medicago sativa
Medical Microbiology
monophyly
Nucleotide sequence
nucleotide sequences
Phylogeny
Plant Sciences
Plant viruses
sequence diversity
Short Report
Virology
Argentina
Geographical distribution
Alfalfa enamovirus-1
Vector transmission
Alfalfa
Genetic diversity
ISSN:0920-8569, 1572-994X, 1572-994X
Online Access:Get full text
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Summary:Alfalfa ( Medicago sativa L.) growing areas of Argentina were surveyed between 2010 and 2018 to determine the geographical distribution and analyse the genetic diversity among alfalfa enamovirus-1 (AEV-1) isolates. The virus was detected in all 17 surveyed alfalfa-producing provinces, with a prevalence of 64%. The plant virus AEV-1 is widely distributed in the country, and its transmission vector has been unknown until now. Here we show that the black aphid Aphis craccivora can transmit AEV-1. The CP sequence identity among 16 AEV-1 isolates from Argentina was from 98 to 100% and from 98.9 to 100% at nucleotide and amino acid levels, respectively, indicating a low level of sequence variation among these isolates. The phylogenetic analysis based on the complete nucleotide sequence of the CP gene indicated that AEV-1 isolates are closely related and clustered in a monophyletic group. These results suggest that AEV-1 has spread very recently in Argentina. In the present study, we report the geographical distribution of AEV-1 in the main alfalfa-growing areas of Argentina and, for the first time, identify an insect vector and describe the CP gene diversity of an enamovirus worldwide.
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ISSN:0920-8569
1572-994X
1572-994X
DOI:10.1007/s11262-020-01783-z