Identification of a phage-derived depolymerase specific for KL64 capsule of Klebsiella pneumoniae and its anti-biofilm effect

The increasing prevalence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a serious threat to global health. Phages and phage-derived enzymes gained increasing attention for controling CRKP infections. In this study, a lytic phage P510 infecting KL64 type K. pneumoniae was isolated and ch...

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Veröffentlicht in:Virus genes Jg. 57; H. 5; S. 434 - 442
Hauptverfasser: Li, Min, Li, Pei, Chen, Long, Guo, Genglin, Xiao, Yuyi, Chen, Liang, Du, Hong, Zhang, Wei
Format: Journal Article
Sprache:Englisch
Veröffentlicht: New York Springer US 01.10.2021
Springer Nature B.V
Schlagworte:
Antibiotics
Autographiviridae
bacteriophages
biofilm
Biofilms
Biomedical and Life Sciences
Biomedicine
Electron microscopy
Genomes
Host range
Klebsiella pneumoniae
Lysis
Medical Microbiology
Original Paper
Phages
Plant Sciences
Polysaccharides
Public health
sequence analysis
tail
Tail fiber protein
Virology
Bacteriophages
Biofilm
Carbapenem-resistant
Depolymerase
Capsular type
ISSN:0920-8569, 1572-994X, 1572-994X
Online-Zugang:Volltext
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Zusammenfassung:The increasing prevalence of Carbapenem-resistant Klebsiella pneumoniae (CRKP) poses a serious threat to global health. Phages and phage-derived enzymes gained increasing attention for controling CRKP infections. In this study, a lytic phage P510 infecting KL64 type K. pneumoniae was isolated and characterized. Whole genome analysis and electron microscopy analysis showed that phage P510 belonged to genus Przondovirus , family Autographiviridae , the order Caudovirales . The tail fiber protein of the phage was predicted to encode capsule depolymerase. Further analysis demonstrated that recombinant depolymerase P510dep had polysaccharide-degrading activity against KL64-types capsule of K. pneumoniae , and its lysis spectrum matched to host range of phage P510. We also demonstrated that the recombinant depolymerase was able to significantly inhibit biofilm formation. The discovery of the phage-derived depolymerase lays the foundation for controlling the spread of CRKPs.
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ISSN:0920-8569
1572-994X
1572-994X
DOI:10.1007/s11262-021-01847-8