DNA methylation profiling and integrative multi-omics analysis of skin samples reveal important contribution of epigenetics and immune response in the pathogenesis of acne vulgaris

The regulatory effect of DNA methylation on the pathogenesis of acne vulgaris is completely unknown. Herein we analyzed the DNA methylation profile in skin samples of acne vulgaris and further integrated it with gene expression profiles and single-cell RNA-sequencing data. Finally, 31,134 differenti...

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Published in:Clinical immunology (Orlando, Fla.) Vol. 255; p. 109773
Main Authors: Liu, Lin, Xue, Yuzhou, Chen, Jiayi, Li, Yuxin, Chen, Tingqiao, Pan, Xingyu, Zhong, Judan, Shao, Xinyi, Chen, Yangmei, Chen, Jin
Format: Journal Article
Language:English
Published: Elsevier Inc 01.10.2023
Subjects:
Acne vulgaris
DNA methylation
Integrative multi-omics analysis
Multi-omics
Acne vulgaris
DNA methylation
Integrative multi-omics analysis
Multi-omics
ISSN:1521-6616, 1521-7035, 1521-7035
Online Access:Get full text
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Summary:The regulatory effect of DNA methylation on the pathogenesis of acne vulgaris is completely unknown. Herein we analyzed the DNA methylation profile in skin samples of acne vulgaris and further integrated it with gene expression profiles and single-cell RNA-sequencing data. Finally, 31,134 differentially methylated sites and 770 differentially methylated and expressed genes (DMEGs) were identified. The multi-omics analysis suggested the importance of DNA methylation in inflammation and immunity in acne. And DMEGs were verified in an external dataset and were closely related to early inflammatory acne. Additionally, we conducted experiments to verify the mRNA expression and DNA methylation level of DMEGs. This study supports the significant contribution of epigenetics to the pathogenesis of acne vulgaris and may provide new ideas for the molecular mechanisms of and potential therapeutic strategies for acne vulgaris. •A total of 31,134 differentially methylated sites were identified on 22 chromosomes.•Eleven key DMEGs were distributed and active in lymphocytes and myeloid cells.•DMEGs were closely related to early inflammatory acne rather than to evolved lesions.
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content type line 23
ISSN:1521-6616
1521-7035
1521-7035
DOI:10.1016/j.clim.2023.109773