Pinkment: a synthetic platform for the development of fluorescent probes for diagnostic and theranostic applications

Reaction-based fluorescent-probes have proven successful for the visualisation of biological species in various cellular processes. Unfortunately, in order to tailor the design of a fluorescent probe to a specific application ( i.e. organelle targeting, material and theranostic applications) often r...

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Published in:Chemical science (Cambridge) Vol. 11; no. 32; pp. 8567 - 8571
Main Authors: Weber, Maria, Han, Hai-Hao, Li, Bo-Han, Odyniec, Maria L., Jarman, Charlotte E. F., Zang, Yi, Bull, Steven D., Mackenzie, Amanda B., Sedgwick, Adam C., Li, Jia, He, Xiao-Peng, James, Tony D.
Format: Journal Article
Language:English
Published: CAMBRIDGE Royal Soc Chemistry 06.08.2020
Royal Society of Chemistry
The Royal Society of Chemistry
Subjects:
Biomarkers
Chemical compounds
Chemistry
Chemistry, Multidisciplinary
Diagnostic software
Diagnostic systems
Fluorescent indicators
Inflammation
Macrophages
Mass spectra
NMR
Nuclear magnetic resonance
Physical Sciences
Science & Technology
Selectivity
Visualization
REACTIVE OXYGEN
ANTIOXIDANTS
PEROXYNITRITE
NITRIC-OXIDE
CLICK-CHEMISTRY
HYDROGEN-PEROXIDE
HEALTH
ISSN:2041-6520, 2041-6539
Online Access:Get full text
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Summary:Reaction-based fluorescent-probes have proven successful for the visualisation of biological species in various cellular processes. Unfortunately, in order to tailor the design of a fluorescent probe to a specific application ( i.e. organelle targeting, material and theranostic applications) often requires extensive synthetic efforts and the synthetic screening of a range of fluorophores to match the required synthetic needs. In this work, we have identified Pinkment-OH as a unique “plug-and-play” synthetic platform that can be used to develop a range of ONOO − responsive fluorescent probes for a variety of applications. These include theranostic-based applications and potential material-based/bioconjugation applications. The as prepared probes displayed an excellent sensitivity and selectivity for ONOO − over other ROS. In vitro studies using HeLa cells and RAW 264.7 macrophages demonstrated their ability to detect exogenously and endogenously produced ONOO − . Evaluation in an LPS-induced inflammation mouse model illustrated the ability to monitor ONOO − production in acute inflammation. Lastly, theranostic-based probes enabled the simultaneous evaluation of indomethacin-based therapeutic effects combined with the visualisation of an inflammation biomarker in RAW 264.7 cells.
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These authors contributed equally to this work.
ISSN:2041-6520
2041-6539
DOI:10.1039/D0SC02438D