Dynamic ray tracing for modeling optical cell manipulation
Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real a...
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| Vydané v: | Optics express Ročník 18; číslo 16; s. 16702 - 16714 |
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| Hlavní autori: | , , , |
| Médium: | Journal Article |
| Jazyk: | English |
| Vydavateľské údaje: |
United States
Optical Society of America
02.08.2010
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| Predmet: | |
| ISSN: | 1094-4087, 1094-4087 |
| On-line prístup: | Získať plný text |
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| Shrnutí: | Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real and non-rigid cells can deform, so assumptions inherent in traditional ray optics methods begin to break down. In this work, we implement a dynamic ray tracing technique to calculate the stress distribution on a deformable cell induced by optical trapping. Here, cells are modeled as three-dimensional elastic capsules with a discretized surface with associated hydrodynamic forces calculated using the Immersed Boundary Method. We use this approach to simulate the transient deformation of spherical, ellipsoidal and biconcave capsules due to external optical forces induced by a single diode bar optical trap for a range of optical powers. |
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| Bibliografia: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 1094-4087 1094-4087 |
| DOI: | 10.1364/OE.18.016702 |