Dynamic ray tracing for modeling optical cell manipulation

Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real a...

Full description

Saved in:
Bibliographic Details
Published in:Optics express Vol. 18; no. 16; pp. 16702 - 16714
Main Authors: Sraj, Ihab, Szatmary, Alex C., Marr, David W. M., Eggleton, Charles D.
Format: Journal Article
Language:English
Published: United States Optical Society of America 02.08.2010
Subjects:
Animals
Cells
Computer Simulation
Hydrodynamics
Micromanipulation - methods
Models, Biological
Optical Tweezers
Skates (Fish)
Stress, Mechanical
ISSN:1094-4087, 1094-4087
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real and non-rigid cells can deform, so assumptions inherent in traditional ray optics methods begin to break down. In this work, we implement a dynamic ray tracing technique to calculate the stress distribution on a deformable cell induced by optical trapping. Here, cells are modeled as three-dimensional elastic capsules with a discretized surface with associated hydrodynamic forces calculated using the Immersed Boundary Method. We use this approach to simulate the transient deformation of spherical, ellipsoidal and biconcave capsules due to external optical forces induced by a single diode bar optical trap for a range of optical powers.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.18.016702