Prime editing in plants and mammalian cells: Mechanism, achievements, limitations, and future prospects

Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been...

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Vydané v:BioEssays Ročník 44; číslo 9; s. e2200032 - n/a
Hlavní autori: Hillary, V. Edwin, Ceasar, S. Antony
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Cambridge Wiley Subscription Services, Inc 01.09.2022
Predmet:
base editing
Cas9 H840 nickase
CRISPR
CRISPR/Cas
Editing
gene editing
genome
Genomes
Mammalian cells
Mammals
Nuclease
pegRNA
plants
prime editing
Recombinase
recombinases
researchers
ISSN:0265-9247, 1521-1878, 1521-1878
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Shrnutí:Clustered, regularly interspaced, short palindromic repeat (CRISPR)/CRISPR‐associated protein (CRISPR/Cas) system has revolutionized genetic research in the life sciences. Four classes of CRISPR/Cas‐derived genome editing agents, such as nuclease, base editor, recombinase, and prime editor have been introduced for engineering the genomes of diverse organisms. The recently introduced prime editing system offers precise editing without many off‐target effects than traditional CRISPR‐based systems. Many researchers have successfully applied this gene‐editing toolbox in diverse systems for various genome‐editing applications. This review presents the mechanism of prime editing and summarizes the details of the prime editing system applied in plants and mammalian cells for precise genome editing. We also discuss the advantages, limitations, and potential future applications of prime editing in these systems. This review enables the researcher to gain knowledge on prime editing tools and their potential applications in plants and mammalian cells. Prime editing is a search‐and‐replace genome editing system, which mediates all possible 12 base‐to‐base conversions without DSBs and donor templates at a targeted sequence. We describe how this prime editing system helps in improving genetic studies in plants and mammalian cells with their mechanism, achievements, limitations, and future prospects.
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ISSN:0265-9247
1521-1878
1521-1878
DOI:10.1002/bies.202200032