Potential for HER-2/neu molecular targeted therapy for invasive bladder carcinoma: comparative study of immunohistochemistry and fluorescent in situ hybridization

Analysis of HER-2/neu expression in invasive bladder carcinoma was performed in order to evaluate the potential for molecular targeted therapy targeting HER-2. The subjects were 40 patients who were pathologically diagnosed with invasive transitional cell carcinoma of the bladder (pT2 to pT4). A Her...

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Vydáno v:Oncology reports Ročník 19; číslo 1; s. 57
Hlavní autoři: Matsubara, Hiroyuki, Yamada, Yoshiaki, Naruse, Katsuya, Nakamura, Kogenta, Aoki, Shigeyuki, Taki, Tomohiro, Tobiume, Motoi, Zennami, Kenji, Katsuda, Remi, Honda, Nobuaki
Médium: Journal Article
Jazyk:angličtina
Vydáno: Greece 01.01.2008
Témata:
Aged
Aged, 80 and over
Biomarkers, Tumor - analysis
Carcinoma, Transitional Cell - metabolism
Carcinoma, Transitional Cell - pathology
Drug Delivery Systems
Female
Gene Amplification
Gene Expression
Humans
Immunohistochemistry
In Situ Hybridization, Fluorescence
Lymphatic Metastasis - pathology
Male
Middle Aged
Receptor, ErbB-2 - biosynthesis
Receptor, ErbB-2 - genetics
Urinary Bladder Neoplasms - metabolism
Urinary Bladder Neoplasms - pathology
ISSN:1021-335X
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Shrnutí:Analysis of HER-2/neu expression in invasive bladder carcinoma was performed in order to evaluate the potential for molecular targeted therapy targeting HER-2. The subjects were 40 patients who were pathologically diagnosed with invasive transitional cell carcinoma of the bladder (pT2 to pT4). A Hercep test kit was used to detect HER-2 expression, and a Path Vysion kit was used for gene amplification. On immunohistochemical (IHC) staining, the primary tumors were HER-2 positive in 17 patients (17/40, 42.5%). According to the classification of grade, one Grade 2 patient (1/3) and 16 Grade 3 patients (16/37) were positive (P=0.99). According to the classification of stage, 12 pT2 patients (12/22, 54.5%), 2 pT3 patients (2/13, 15.3%), and 3 pT4 patients (3/5, 60%) were positive (P=0.55). Lymph node metastasis was found in 10 patients, and 3 pN2 patients were HER-2 positive (3/6, 50%) (P=0.32). A statistically significant difference was observed between HER-2-positive primary tumors and metastatic lymph nodes (P=0.02). In fluorescent in situ hybridization (FISH), HER-2/neu gene amplification was detected in the primary tumors in 5 patients (5/40, 12.5%). In all these patients, IHC staining was determined as 3+. Lymph node metastasis was found in 3 pN2 patients (3/6) (P=0.32), and in these patients with HER-2/neu gene-amplified metastatic lymph nodes, the primary tumors were also positive for gene amplification (P=0.02). In these cases, IHC staining was 3+ as well. The concordance rate of IHC-positive cases with cases positive for HER-2/neu gene amplification in FISH was 12.5% (5/40), and the concordance rate of IHC 3+ and gene amplification was 71%. This result suggests that, at present, patients who may potentially benefit from molecular targeted therapy targeting HER-2/neu for invasive bladder carcinoma should be identified by gene amplification analysis using FISH in IHC 3+ patients. In addition, it suggested that efficacy of molecular targeted therapy can be expected even for patients with metastatic lymph nodes as long as the primary tumors are positive for HER-2 expression.
Bibliografie:ObjectType-Article-2
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content type line 23
ISSN:1021-335X
DOI:10.3892/or.19.1.57