Genome-wide identification and expression analyses of the calmodulin and calmodulin-like proteins reveal their involvement in stress response and fruit ripening in papaya

•41 CaM and CML proteins were identified in the papaya genome.•Bioinformatics analysis showed that they were typical CaM and CML in plant.•Expression analysis showed that CaM and CML were involved in stress responses.•Some CaM/CMLs were rigorously regulated by ethylene (ethephon and 1-MCP).•CaM/CMLs...

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Vydáno v:Postharvest biology and technology Ročník 143; s. 13 - 27
Hlavní autoři: Ding, Xiaochun, Zhang, Linpeng, Hao, Yanwei, Xiao, Shuangling, Wu, Zhengxian, Chen, Weixin, Li, Xueping, Zhu, Xiaoyang
Médium: Journal Article
Jazyk:angličtina
Vydáno: Amsterdam Elsevier B.V 01.09.2018
Elsevier BV
Témata:
1-methylcyclopropene
calcium
Calcium ions
Calcium-binding protein
Calmodulin
Calmodulin-like protein
cold stress
Developmental stages
EF-hand
ethephon
ethylene
Fruit ripening
fruiting
Fruits
Gene expression
Gene families
Genes
Genomes
High temperature
Light effects
Low temperature
Nucleotide sequence
Papaya
Phylogeny
Plant growth
promoter regions
Proteins
Ripening
sequence alignment
sequence diversity
Storage conditions
Stress response
Stresses
temperature
Temperature effects
Transcription
transcription (genetics)
Calmodulin-like protein
Fruit ripening
Papaya
Gene expression
Stress response
Calmodulin
ISSN:0925-5214, 1873-2356
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Shrnutí:•41 CaM and CML proteins were identified in the papaya genome.•Bioinformatics analysis showed that they were typical CaM and CML in plant.•Expression analysis showed that CaM and CML were involved in stress responses.•Some CaM/CMLs were rigorously regulated by ethylene (ethephon and 1-MCP).•CaM/CMLs are involved in fruit ripening. Calcium (Ca2+) is an important second messenger involved in diverse developmental and adaptive processes in plants. Calmodulin (CaM) and calmodulin-like (CML) proteins are primary Ca2+ sensors that control diverse cellular functions. In this study, 41 genes encoding CaM and CML proteins were identified in the papaya genome, three of which were CaM and others were CML. Sequence alignment, gene structural and phylogenetic analyses revealed that all CaM/CMLs contained the EF-hand, but not other functional domains, and CaM proteins were quite conservative while CML proteins exhibited sequence diversity and structural multiformity. Promoter analysis identified different types of cis-elements related to plant growth and development, hormone response, light response, stress response and transcriptional enhancement in promoter regions of CpCaM/CML genes. Gene expression analysis showed distinctive expression profiles of the CaM/CMLs in different tissue types, different fruit developmental stages and different fruit storage conditions. Several groups of CaM/CML genes were positively or negatively regulated by high and low temperature stresses, such as CML16, CML17.1, CML24 and CML36, indicating that they may play a role in temperature stress adaption. Notably, some CaM/CML genes were rigorously regulated by ethylene (ethephon and 1-MCP treatment), either in a positive or a negative manner, such as CaM7, CML15, CML16, CML17.1, CML37 and CML46. All these results indicated that CaM and CML gene families might play important roles in fruit development, in response to temperature stresses and in fruit ripening process.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0925-5214
1873-2356
DOI:10.1016/j.postharvbio.2018.04.010