Populus homeobox gene ARBORKNOX1 reveals overlapping mechanisms regulating the shoot apical meristem and the vascular cambium

Secondary growth is supported by a dividing population of meristematic cells within the vascular cambium whose daughter cells are recruited to differentiate within secondary phloem and xylem tissues. We cloned a Populus Class 1 KNOX homeobox gene, ARBORKNOX1 (ARK1), which is orthologous to Arabidops...

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Vydané v:	Plant molecular biology Ročník 61; číslo 6; s. 917 - 932
Hlavní autori:	Groover, A.T, Mansfield, S.D, DiFazio, S.P, Dupper, G, Fontana, J.R, Millar, R, Wang, Y
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Netherlands Springer Nature B.V 01.08.2006
Predmet:	Amino Acid Sequence analysis apical meristems Arabidopsis cambium cell adhesion Cell adhesion & migration Cell differentiation Cell Wall Cell Wall - chemistry Cell Wall - genetics cell walls Cells chemistry Cloning, Molecular DNA, Complementary DNA, Complementary - chemistry DNA, Complementary - genetics extracellular matrix Gene expression gene expression regulation Gene Expression Regulation, Developmental Gene Expression Regulation, Plant gene overexpression genetics growth & development Homeodomain Proteins Homeodomain Proteins - genetics homeotic genes In Situ Hybridization internodes Leaves lignin Lignin - analysis mechanism of action Meristem Meristem - genetics Meristem - growth & development Meristem - ultrastructure methods microarray technology Microscopy, Electron, Scanning Mode of action Molecular Sequence Data Oligonucleotide Array Sequence Analysis Oligonucleotide Array Sequence Analysis - methods phenotype Phylogeny Plant Leaves Plant Leaves - growth & development Plant Leaves - ultrastructure Plant Proteins Plant Proteins - genetics Plant Structures Plant Structures - genetics Plant Structures - growth & development Plant tissues Plants, Genetically Modified Populus Populus - genetics Populus - growth & development Populus - ultrastructure proteins secondary phloem Sequence Analysis, DNA Sequence Homology, Amino Acid shoots Stems ultrastructure xylem
ISSN:	0167-4412, 1573-5028
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Popis
Shrnutí:	Secondary growth is supported by a dividing population of meristematic cells within the vascular cambium whose daughter cells are recruited to differentiate within secondary phloem and xylem tissues. We cloned a Populus Class 1 KNOX homeobox gene, ARBORKNOX1 (ARK1), which is orthologous to Arabidopsis SHOOT MERISTEMLESS (STM). ARK1 is expressed in the shoot apical meristem (SAM) and the vascular cambium, and is down-regulated in the terminally differentiated cells of leaves and secondary vascular tissues that are derived from these meristems. Transformation of Populus with either ARK1 or STM over-expression constructs results in similar morphological phenotypes characterized by inhibition of the differentiation of leaves, internode elongation, and secondary vascular cell types in stems. Microarray analysis showed that 41% of genes up-regulated in the stems of ARK1 over-expressing plants encode proteins involved in extracellular matrix synthesis or modification, including proteins involved in cell identity and signaling, cell adhesion, or cell differentiation. These gene expression differences are reflected in alterations of cell wall biochemistry and lignin composition in ARK1 over-expressing plants. Our results suggest that ARK1 has a complex mode of action that may include regulating cell fates through modification of the extracellular matrix. Our findings support the hypothesis that the SAM and vascular cambium are regulated by overlapping genetic programs.
Bibliografia:	http://dx.doi.org/10.1007/s11103-006-0059-y ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23
ISSN:	0167-4412 1573-5028
DOI:	10.1007/s11103-006-0059-y