Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance

Salinity is an environmental stress that causes decline in crop yield. and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from , , and its putative ortholog in Arabidopsis...

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Published in:Plant physiology (Bethesda) Vol. 184; no. 4; p. 2199
Main Authors: Krishnamurthy, Pannaga, Vishal, Bhushan, Ho, Wan Jing, Lok, Felicia Chien Joo, Lee, Felicia Si Min, Kumar, Prakash P
Format: Journal Article
Language:English
Published: United States 01.12.2020
Subjects:
Avicennia - genetics
Avicennia - physiology
Cell Death - genetics
Cytochrome P-450 Enzyme System - genetics
Cytochrome P-450 Enzyme System - physiology
Gene Expression Regulation, Plant
Genes, Plant
Oryza - genetics
Oryza - physiology
Plant Roots - genetics
Plant Roots - physiology
Salinity
Salt Tolerance - genetics
Salt Tolerance - physiology
Stress, Physiological - physiology
Transcription Factors - genetics
Transcription Factors - physiology
ISSN:1532-2548, 1532-2548
Online Access:Get more information
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Summary:Salinity is an environmental stress that causes decline in crop yield. and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from , , and its putative ortholog in Arabidopsis ( ), , which are involved in apoplastic barrier formation. Both genes were induced by 30 min of salt treatment in the roots. Heterologous expression of in the Arabidopsis mutant and wild-type rice ( ) conferred increased NaCl tolerance to seedlings by enhancing root suberin deposition. Histochemical staining and gas chromatography-tandem mass spectrometry quantification of suberin precursors confirmed the role of CYP94B1 in suberin biosynthesis. Using chromatin immunoprecipitation and yeast one-hybrid and luciferase assays, we identified AtWRKY33 as the upstream regulator of in Arabidopsis. In addition, mutants exhibited reduced suberin and salt-sensitive phenotypes, which were rescued by expressing in the background. This further confirmed that AtWRKY33-mediated regulation of is part of the salt tolerance mechanism. Our findings may help efforts aimed at generating salt-tolerant crops.
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ISSN:1532-2548
1532-2548
DOI:10.1104/pp.20.01054