Regulation of a Cytochrome P450 Gene CYP94B1 by WRKY33 Transcription Factor Controls Apoplastic Barrier Formation in Roots to Confer Salt Tolerance

Salinity is an environmental stress that causes decline in crop yield. and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from , , and its putative ortholog in Arabidopsis...

Celý popis

Uloženo v:

Podrobná bibliografie
Vydáno v:	Plant physiology (Bethesda) Ročník 184; číslo 4; s. 2199
Hlavní autoři:	Krishnamurthy, Pannaga, Vishal, Bhushan, Ho, Wan Jing, Lok, Felicia Chien Joo, Lee, Felicia Si Min, Kumar, Prakash P
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	United States 01.12.2020
Témata:	Avicennia - genetics Avicennia - physiology Cell Death - genetics Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - physiology Gene Expression Regulation, Plant Genes, Plant Oryza - genetics Oryza - physiology Plant Roots - genetics Plant Roots - physiology Salinity Salt Tolerance - genetics Salt Tolerance - physiology Stress, Physiological - physiology Transcription Factors - genetics Transcription Factors - physiology
ISSN:	1532-2548, 1532-2548
On-line přístup:	Zjistit podrobnosti o přístupu
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!

Popis
Shrnutí:	Salinity is an environmental stress that causes decline in crop yield. and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from , , and its putative ortholog in Arabidopsis ( ), , which are involved in apoplastic barrier formation. Both genes were induced by 30 min of salt treatment in the roots. Heterologous expression of in the Arabidopsis mutant and wild-type rice ( ) conferred increased NaCl tolerance to seedlings by enhancing root suberin deposition. Histochemical staining and gas chromatography-tandem mass spectrometry quantification of suberin precursors confirmed the role of CYP94B1 in suberin biosynthesis. Using chromatin immunoprecipitation and yeast one-hybrid and luciferase assays, we identified AtWRKY33 as the upstream regulator of in Arabidopsis. In addition, mutants exhibited reduced suberin and salt-sensitive phenotypes, which were rescued by expressing in the background. This further confirmed that AtWRKY33-mediated regulation of is part of the salt tolerance mechanism. Our findings may help efforts aimed at generating salt-tolerant crops.
Bibliografie:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1532-2548 1532-2548
DOI:	10.1104/pp.20.01054