Emerging mass spectrometry-based proteomics methodologies for novel biomedical applications

Research into the basic biology of human health and disease, as well as translational human research and clinical applications, all benefit from the growing accessibility and versatility of mass spectrometry (MS)-based proteomics. Although once limited in throughput and sensitivity, proteomic studie...

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Veröffentlicht in:Biochemical Society transactions Jg. 48; H. 5; S. 1953
Hauptverfasser: Pino, Lindsay K, Rose, Jacob, O'Broin, Amy, Shah, Samah, Schilling, Birgit
Format: Journal Article
Sprache:Englisch
Veröffentlicht: England 30.10.2020
Schlagworte:
Animals
Chromatography
Humans
Inflammation
Isotopes
Mass Spectrometry - methods
Mice
Precision Medicine
Protein Interaction Mapping
Protein Processing, Post-Translational
Proteins - chemistry
Proteome - metabolism
Proteomics - methods
Signal Transduction
Translational Research, Biomedical
Workflow
proteomics
mass spectrometry
technology
ISSN:1470-8752, 1470-8752
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Zusammenfassung:Research into the basic biology of human health and disease, as well as translational human research and clinical applications, all benefit from the growing accessibility and versatility of mass spectrometry (MS)-based proteomics. Although once limited in throughput and sensitivity, proteomic studies have quickly grown in scope and scale over the last decade due to significant advances in instrumentation, computational approaches, and bio-sample preparation. Here, we review these latest developments in MS and highlight how these techniques are used to study the mechanisms, diagnosis, and treatment of human diseases. We first describe recent groundbreaking technological advancements for MS-based proteomics, including novel data acquisition techniques and protein quantification approaches. Next, we describe innovations that enable the unprecedented depth of coverage in protein signaling and spatiotemporal protein distributions, including studies of post-translational modifications, protein turnover, and single-cell proteomics. Finally, we explore new workflows to investigate protein complexes and structures, and we present new approaches for protein-protein interaction studies and intact protein or top-down MS. While these approaches are only recently incipient, we anticipate that their use in biomedical MS proteomics research will offer actionable discoveries for the improvement of human health.
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ISSN:1470-8752
1470-8752
DOI:10.1042/BST20191091