Tissue-specific features of the T cell repertoire after allogeneic hematopoietic cell transplantation in human and mouse

T cells are the central drivers of many inflammatory diseases, but the repertoire of tissue-resident T cells at sites of pathology in human organs remains poorly understood. We examined the site-specificity of T cell receptor (TCR) repertoires across tissues (5 to 18 tissues per patient) in prospect...

Celý popis

Uložené v:

Podrobná bibliografia
Vydané v:	Science translational medicine Ročník 15; číslo 706; s. eabq0476
Hlavní autori:	DeWolf, Susan, Elhanati, Yuval, Nichols, Katherine, Waters, Nicholas R, Nguyen, Chi L, Slingerland, John B, Rodriguez, Natasia, Lyudovyk, Olga, Giardina, Paul A, Kousa, Anastasia I, Andrlová, Hana, Ceglia, Nick, Fei, Teng, Kappagantula, Rajya, Li, Yanyun, Aleynick, Nathan, Baez, Priscilla, Murali, Rajmohan, Hayashi, Akimasa, Lee, Nicole, Gipson, Brianna, Rangesa, Madhumitha, Katsamakis, Zoe, Dai, Anqi, Blouin, Amanda G, Arcila, Maria, Masilionis, Ignas, Chaligne, Ronan, Ponce, Doris M, Landau, Heather J, Politikos, Ioannis, Tamari, Roni, Hanash, Alan M, Jenq, Robert R, Giralt, Sergio A, Markey, Kate A, Zhang, Yanming, Perales, Miguel-Angel, Socci, Nicholas D, Greenbaum, Benjamin D, Iacobuzio-Donahue, Christine A, Hollmann, Travis J, van den Brink, Marcel R M, Peled, Jonathan U
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	United States 26.07.2023
Predmet:	Animals Graft vs Host Disease - pathology Hematopoietic Stem Cell Transplantation Humans Mice Receptors, Antigen, T-Cell T-Lymphocytes - pathology
ISSN:	1946-6242, 1946-6242
On-line prístup:	Zistit podrobnosti o prístupe
Tagy:	Pridať tag Žiadne tagy, Buďte prvý, kto otaguje tento záznam!

Popis
Shrnutí:	T cells are the central drivers of many inflammatory diseases, but the repertoire of tissue-resident T cells at sites of pathology in human organs remains poorly understood. We examined the site-specificity of T cell receptor (TCR) repertoires across tissues (5 to 18 tissues per patient) in prospectively collected autopsies of patients with and without graft-versus-host disease (GVHD), a potentially lethal tissue-targeting complication of allogeneic hematopoietic cell transplantation, and in mouse models of GVHD. Anatomic similarity between tissues was a key determinant of TCR repertoire composition within patients, independent of disease or transplant status. The T cells recovered from peripheral blood and spleens in patients and mice captured a limited portion of the TCR repertoire detected in tissues. Whereas few T cell clones were shared across patients, motif-based clustering revealed shared repertoire signatures across patients in a tissue-specific fashion. T cells at disease sites had a tissue-resident phenotype and were of donor origin based on single-cell chimerism analysis. These data demonstrate the complex composition of T cell populations that persist in human tissues at the end stage of an inflammatory disorder after lymphocyte-directed therapy. These findings also underscore the importance of studying T cell in tissues rather than blood for tissue-based pathologies and suggest the tissue-specific nature of both the endogenous and posttransplant T cell landscape.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1946-6242 1946-6242
DOI:	10.1126/scitranslmed.abq0476