Dissemination of antibiotic-resistant bacteria associated with microplastics collected from Monastir and Mahdia coasts (Tunisia)

The exponential use of plastics and their recalcitrant nature leads to their significant accumulation in the environment. The occurrence of plastic wastes is considered as a serious environmental problem. Additionally, plastic wastes can break down into smaller pieces called microplastics (MPs), lea...

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Vydáno v:Microbial pathogenesis Ročník 198; s. 107193
Hlavní autoři: Hassen, Wafa, Danioux, Anthony, Oueslati, Amira, Santana-Rodríguez, José Juan, Sire, Olivier, Sedrati, Mouncef, Ben Mansour, Hedi
Médium: Journal Article
Jazyk:angličtina
Vydáno: England Elsevier Ltd 01.01.2025
Elsevier
Témata:
Acinetobacter
Acinetobacter johnsonii
Aeromonas
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Bacteria - classification
Bacteria - drug effects
Bacteria - genetics
Bacteria - isolation & purification
benzylpenicillin
catalase
DNA, Bacterial - genetics
Drug Resistance, Bacterial
Enterobacter
Environmental impact
Environmental Sciences
Exiguobacterium
genus
haplotypes
Internal transcribed spacer
internal transcribed spacers
Microbial Sensitivity Tests
Microplastic-associated bacterial isolates
Microplastics
Microplastics (MPs)
multiple drug resistance
pathogenesis
Phylogeny
Pseudomonas
ribosomal DNA
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Shewanella
species
Staphylococcus
Tunisia
Vibrio
Tunisia
Acinetobacter
Internal transcribed spacer
Microplastics (MPs)
Environmental impact
Microplastic-associated bacterial isolates
Antibiotic resistance
ISSN:0882-4010, 1096-1208, 1096-1208
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Shrnutí:The exponential use of plastics and their recalcitrant nature leads to their significant accumulation in the environment. The occurrence of plastic wastes is considered as a serious environmental problem. Additionally, plastic wastes can break down into smaller pieces called microplastics (MPs), leading to further interactions with the environment and living organisms. In this study, sixty-six strains were isolated from microplastic particles collected on different coastal areas of Monastir and Mahdia (Tunisia). The different bacterial isolates were identified according to some biochemical tests such as catalase, oxidase, and were subjected to molecular characterization. Amplification of the internal transcribed spacer (ITS) revealed the presence of 31 ITS haplotypes. The partial sequencing of the 16S ribosomal DNA of representative strains was analyzed. The majority of bacterial isolates (84.31 %) belonged to Gamma-proteobacteria (84.78 %), while the remaining isolates were affiliated to Firmicutes (15.21 %). The microplastic-associated bacterial isolates belonged to 10 genera, namely Acinetobacter, Pseudomonas, Bacillus, Staphylococcus, Shewanella, Aeromonas, Vibrio, Stutzerimonas, Exiguobacterium, Enterobacter. Among the well-represented Acinetobacter genus, the most common species identified was Acinetobacter johnsonii. Susceptibility patterns of these strains were studied against 21 antibiotics commonly used in Tunisia. A high level of antibiotic resistance was observed for Penicillin G (97.82 %) and Temocillin (86.95 %). S26 strain presented the highest multidrug resistance with a multiple antibiotic resistance (MAR) index of 0.71. [Display omitted] •Microplastics have been extracted from sediment of different coastal areas using the density separation method.•Sixty-six strains were isolated from collected microplastic particles and identified by 16S-rRNA sequencing.•Bacterial isolates belonged to Gamma-proteobacteria and Firmicutes and the most commonly identified genus was Acinetobacter.•A high level of antibiotic resistance was observed for Penicillin G and Temocillin.
Bibliografie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0882-4010
1096-1208
1096-1208
DOI:10.1016/j.micpath.2024.107193