Spatial Distribution of Protein and Phenolic Constituents in Wheat Grain as Probed by Confocal Raman Microspectroscopy

Confocal Raman microspectroscopy is an appropriate method to investigate the microscopic structure of a wheat grain. The technique does not require any destructive preparation of the sample and is rapidly performed. The high spatial resolution reveals molecular and chemical heterogeneity within cell...

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Veröffentlicht in:Journal of cereal science Jg. 32; H. 1; S. 57 - 71
Hauptverfasser: Piot, O., Autran, J.-C., Manfait, M.
Format: Journal Article
Sprache:Englisch
Veröffentlicht: Kidlington Elsevier Ltd 01.07.2000
Elsevier
Schlagworte:
aleurone cells
arabinoxylan
Biological and medical sciences
cell walls
Cereal and baking product industries
endosperm
ferulic acid
Food engineering
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
hardness
Life Sciences
Methods of analysis, processing and quality control, regulation, standards
plant proteins
protein content
Raman microspectroscopy, Triticum aestivum wheat, hardness, structure, phenolic acid, protein, starch
seed development
spatial distribution
spectroscopy
starch granules
Triticum aestivum
varieties
wheat
xylan
Raman microspectroscopy, Triticum aestivum wheat, hardness, structure, phenolic acid, protein, starch
Monocotyledones
Grains
Proximate analysis
Confocal microscopy
Hardness
Cereal crop
Soft wheat
Glucan
Spatial distribution
Gramineae
Angiospermae
Analytical method
Phenols
Cereal
Microscopic structure
Aromatic compound
Spermatophyta
Property structure relationship
Raman spectrometry
Wheat starch
Physicochemical properties
Protein content
Triticum aestivum
STRUCTURE
ISSN:0733-5210, 1095-9963
Online-Zugang:Volltext
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Zusammenfassung:Confocal Raman microspectroscopy is an appropriate method to investigate the microscopic structure of a wheat grain. The technique does not require any destructive preparation of the sample and is rapidly performed. The high spatial resolution reveals molecular and chemical heterogeneity within cellular dimensions. Using specific vibration bands as markers, the technique permits reconstruction of spectral images. The different components of wheat ( Triticum aestivum) were chemically and structurally characterised by Raman microspectroscopy. The work was focused on the protein content and composition of the starchy endosperm and on the composition of the aleurone cells walls in arabinoxylan and ferulic acid derivatives. Particular attention was given to these components because of their role in cohesion of the starch–protein matrix interface and of the endosperm–envelope interface. Confocal Raman microscopy was also used to follow the evolution of protein content and structure during grain development of various wheat varieties selected on the basis of hardness level and aptitude to separation of peripheral layers during milling.
Bibliographie:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0733-5210
1095-9963
DOI:10.1006/jcrs.2000.0314