Spatial Distribution of Protein and Phenolic Constituents in Wheat Grain as Probed by Confocal Raman Microspectroscopy

Confocal Raman microspectroscopy is an appropriate method to investigate the microscopic structure of a wheat grain. The technique does not require any destructive preparation of the sample and is rapidly performed. The high spatial resolution reveals molecular and chemical heterogeneity within cell...

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Vydané v:	Journal of cereal science Ročník 32; číslo 1; s. 57 - 71
Hlavní autori:	Piot, O., Autran, J.-C., Manfait, M.
Médium:	Journal Article
Jazyk:	English
Vydavateľské údaje:	Kidlington Elsevier Ltd 01.07.2000 Elsevier
Predmet:	aleurone cells arabinoxylan Biological and medical sciences cell walls Cereal and baking product industries endosperm ferulic acid Food engineering Food industries Fundamental and applied biological sciences. Psychology General aspects hardness Life Sciences Methods of analysis, processing and quality control, regulation, standards plant proteins protein content Raman microspectroscopy, Triticum aestivum wheat, hardness, structure, phenolic acid, protein, starch seed development spatial distribution spectroscopy starch granules Triticum aestivum varieties wheat xylan Raman microspectroscopy, Triticum aestivum wheat, hardness, structure, phenolic acid, protein, starch Monocotyledones Grains Proximate analysis Confocal microscopy Hardness Cereal crop Soft wheat Glucan Spatial distribution Gramineae Angiospermae Analytical method Phenols Cereal Microscopic structure Aromatic compound Spermatophyta Property structure relationship Raman spectrometry Wheat starch Physicochemical properties Protein content Triticum aestivum STRUCTURE
ISSN:	0733-5210, 1095-9963
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Shrnutí:	Confocal Raman microspectroscopy is an appropriate method to investigate the microscopic structure of a wheat grain. The technique does not require any destructive preparation of the sample and is rapidly performed. The high spatial resolution reveals molecular and chemical heterogeneity within cellular dimensions. Using specific vibration bands as markers, the technique permits reconstruction of spectral images. The different components of wheat ( Triticum aestivum) were chemically and structurally characterised by Raman microspectroscopy. The work was focused on the protein content and composition of the starchy endosperm and on the composition of the aleurone cells walls in arabinoxylan and ferulic acid derivatives. Particular attention was given to these components because of their role in cohesion of the starch–protein matrix interface and of the endosperm–envelope interface. Confocal Raman microscopy was also used to follow the evolution of protein content and structure during grain development of various wheat varieties selected on the basis of hardness level and aptitude to separation of peripheral layers during milling.
Bibliografia:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0733-5210 1095-9963
DOI:	10.1006/jcrs.2000.0314