Development of a Polymerase Chain Reaction Protocol for Detection of Xylella fastidiosa in Plant Tissue

A 7.4-kb RI fragment of genomic DNA of strain PCE-RR (ATCC 35879) was used as a probe and was conserved in 18 strains of . The nucleotide sequence of a 1.0-kb internal RV portion of the fragment was determined, and oligonucleotides were selected for primers that amplified genomic DNA specific to in...

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Bibliographic Details

Published in:	Phytopathology Vol. 115; no. 8V; p. 456
Main Authors:	Minsavage, G V, Thompson, C M, Hopkins, D L, Leite, R M V B C, Stall, R E
Format:	Journal Article
Language:	English
Published:	United States 01.08.2025
Subjects:	Citrus - microbiology DNA, Bacterial - genetics Enzyme-Linked Immunosorbent Assay Plant Diseases - microbiology Polymerase Chain Reaction - methods Sensitivity and Specificity Vitis - microbiology Xylella - genetics Xylella - isolation & purification plum leaf scald citrus variegated chlorosis Pierce's disease of grapevine
ISSN:	0031-949X
Online Access:	Get more information
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