Molecular structure of the allelic variants of (AAT)n microsatellite locus Du47D in the parthenogenetic species Darevskia unisexualis and bisexual parental species D. valentini and D. raddei

Microsatellite repeats are one of the most widespread elements of the eukaryotic genome, but are poorly studied in species with clonal reproduction. PCR analysis and DNA sequencing were used to study the molecular structure of the allelic variants of microsatellite locus Du47D in the parthenogenetic...

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Vydané v:Russian journal of genetics Ročník 46; číslo 5; s. 630 - 632
Hlavní autori: Korchagin, V. I, Tokarskaya, O. N
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01.05.2010
SP MAIK Nauka/Interperiodica
Predmet:
alleles
ancestry
Animal Genetics and Genomics
asexual reproduction
Biomedical and Life Sciences
Biomedicine
chemical structure
DNA
Human Genetics
Lacerta
Lacerta saxicola
loci
Microbial Genetics and Genomics
microsatellite repeats
parthenogenesis
polymerase chain reaction
sequence analysis
Short Communications
Stepwise Mutation Model
Microsatellite Locus
Parthenogenetic Species
Lacertid Lizard
Bisexual Species
ISSN:1022-7954, 1608-3369
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Shrnutí:Microsatellite repeats are one of the most widespread elements of the eukaryotic genome, but are poorly studied in species with clonal reproduction. PCR analysis and DNA sequencing were used to study the molecular structure of the allelic variants of microsatellite locus Du47D in the parthenogenetic species Darevskia unisexualis and its evolutionary ancestors, bisexual species D. raddei and D. valentini, of the genus Darevskia (Lacerta saxicola complex). Sequencing showed that the allelic variants of the D. unisexualis Du47D locus and the alleles of its D. raddei and D. valentini orthologs have a perfect microsatellite cluster structure, differ in number of ATT monomeric units, and have certain species-specific combinations of nucleotide substitutions, deletions, and insertions in the microsatellite-flanking DNA sequences. The Du47D alleles that the parthenogenetic species inherited from D. valentini or from D. raddei were identified.
Bibliografia:http://dx.doi.org/10.1134/S1022795410050182
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ISSN:1022-7954
1608-3369
DOI:10.1134/S1022795410050182