Effect of water on methanolysis of glycerol trioleate catalyzed by immobilized lipase Candida sp. 99–125 in organic solvent system

In this study, hydrolysis and methanolysis of glycerol trioleate (TG) by lipase Candida sp. 99–125 were investigated under different water conditions. Both the reaction rates were relatively low without water, while increasing water content to 5 wt.% (or more, from 10–20%) based on the TG amount cau...

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Vydáno v:Journal of molecular catalysis. B, Enzymatic Ročník 56; číslo 2; s. 122 - 125
Hlavní autoři: Lu, Jike, Chen, Yawei, Wang, Fang, Tan, Tianwei
Médium: Journal Article Konferenční příspěvek
Jazyk:angličtina
Vydáno: Amsterdam Elsevier B.V 01.02.2009
Elsevier
Témata:
Bioconversions. Hemisynthesis
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Glycerol trioleate
Hydrolysis
Immobilized lipase
Methanolysis
Methods. Procedures. Technologies
Water
Water
Hydrolysis
Glycerol trioleate
Immobilized lipase
Methanolysis
Yeast
Enzyme
Glycerol
Triacylglycerol lipase
Esterases
Carboxylic ester hydrolases
Fungi
Organic solvent
Candida
Hydrolases
Fungi Imperfecti
Catalysis
ISSN:1381-1177, 1873-3158
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Shrnutí:In this study, hydrolysis and methanolysis of glycerol trioleate (TG) by lipase Candida sp. 99–125 were investigated under different water conditions. Both the reaction rates were relatively low without water, while increasing water content to 5 wt.% (or more, from 10–20%) based on the TG amount caused remarkable higher TG conversion for both reactions. Moreover, comparing the time course curves of the hydrolysis and methanolysis, it could be concluded that the methanolysis reaction catalyzed by this Candida sp. 99–125 appeared to accord with the successive reaction mechanism. TG was first hydrolyzed to partial glycerides and oleic acid (OA), then oleic acid methyl ester (OAME) was produced by esterification of the OA with methanol. This water effect was also confirmed by the experiments that water substitutions such as t-butanol and some surfactants added into the system did not get such high yields as that of the water included system. So these results showed that water took part in the methanolysis reaction, and successive hydrolysis–esterification process might be the catalytic mechanism of this lipase.
ISSN:1381-1177
1873-3158
DOI:10.1016/j.molcatb.2008.05.004