Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement

Purpose Platelet-rich plasma (PRP) is a remarkable substance, which involves the growth and proliferation of all cell types. As a source of growth factors, we evaluated whether sperm cryopreservation supplemented with PRP improves the rates of sperm motility, viability, and DNA integrity after vitri...

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Vydané v:Cell and tissue banking Ročník 24; číslo 2; s. 307 - 315
Hlavní autori: Nabavinia, Maryam Sadat, Yari, Arash, Ghasemi-Esmailabad, Saeed, Gholoobi, Aida, Gholizadeh, Lida, Nabi, Ali, Lotfi, Marzieh, Khalili, Mohammad Ali
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Dordrecht Springer Netherlands 01.06.2023
Springer Nature B.V
Predmet:
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cryopreservation
Cryoprotective Agents - pharmacology
Cryoprotectors
Dietary Supplements
Freezing
Full Length Paper
Growth factors
Humans
Life Sciences
Male
Motility
Platelet-Rich Plasma
Platelets
Semen
Sperm
Sperm Motility
Spermatozoa
Transplant Surgery
Viability
Vitrification
Cryoprotectant medium
Sperm parameters
Platelet-rich plasma
Vitrification
ISSN:1389-9333, 1573-6814, 1573-6814
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Shrnutí:Purpose Platelet-rich plasma (PRP) is a remarkable substance, which involves the growth and proliferation of all cell types. As a source of growth factors, we evaluated whether sperm cryopreservation supplemented with PRP improves the rates of sperm motility, viability, and DNA integrity after vitrification compared with conventional cryo-medium. Materials and methods 20 normal semen specimens were collected from healthy men. After swim-up preparation, each sample was divided into four aliquots. One, as control, received no treatment, and the other three experimental samples were treated with three different concentrations of PRP as cryoprotectant. Sperm parameters were examined before and after freezing procedure. Results PRP had no significant effect on sperm count. Meanwhile, the percentage of sperm progressive motility and viability in the PRP treated samples with 1×10 5 /µL concentration was significantly higher than control group. Besides, the rate of immotile sperms in these samples was significantly lower than the control. Sperm viability was significantly higher in the PRP samples at 1×10 5 /µL concentration. In the case of DNA integrity, CMA3 staining showed that the lower PRP concentration was correlated with the higher rate of abnormal spermatozoa. SCD showed that the rate of abnormal sperms in the PRP samples with 1×10 5 /µL concentration was significantly lower than control group. Conclusions This study showed a protective effect of PRP on human sperm quality at an optimized concentration after vitrification. Besides, the effects of PRP supplementation of sperms on successful fertility following sperm preservation will be of interest.
Bibliografia:ObjectType-Article-1
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ISSN:1389-9333
1573-6814
1573-6814
DOI:10.1007/s10561-022-10032-6