Bone tissue regeneration by 58S bioactive glass scaffolds containing exosome: an in vivo study

Exosomes, the naturally secreted nanocarriers of cells, have recently been demonstrated to have therapeutic benefits in a variety of disease models where parent cells are not present. However, the use of exosomes in bone defect regeneration has been unusual, and little is documented about the underl...

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Vydané v:Cell and tissue banking Ročník 25; číslo 1; s. 389 - 400
Hlavní autori: Ranjbar, Faezeh Esmaeili, Ranjbar, Afsaneh Esmaeili, Malekshahi, Ziba Veisi, Taghdiri-Nooshabadi, Zahra, Faradonbeh, Davood Rabiei, Youseflee, Pouya, Ghasemi, Sahar, Vatanparast, Mahboubeh, Azim, Fazli, Nooshabadi, Vajihe Taghdiri
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Dordrecht Springer Netherlands 01.03.2024
Springer Nature B.V
Predmet:
Biological activity
Biomedical and Life Sciences
Biomedicine
Bone growth
Bone implants
Bones
Calvaria
Cell Biology
Cell culture
Computed tomography
Exosomes
Full Length Paper
Immunohistochemistry
Life Sciences
Osteogenesis
Porosity
Regeneration
Stromal cells
Tissue engineering
Transplant Surgery
Bone tissue engineering
Human endometrial mesenchymal stem cells
Exosome
Bioactive glass scaffold
ISSN:1389-9333, 1573-6814, 1573-6814
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Shrnutí:Exosomes, the naturally secreted nanocarriers of cells, have recently been demonstrated to have therapeutic benefits in a variety of disease models where parent cells are not present. However, the use of exosomes in bone defect regeneration has been unusual, and little is documented about the underlying processes. In recent study we produced and characterized exosomes derived human endometrial mesenchymal stem stromal cells and 58S bioactive glass scaffolds; in following, in this research exosome loaded scaffolds synthetized and release of exosome, porosity and bioactivity of them were assessed. More over the effect of scaffolds on repair of critical-size bone defects in rat’s calvaria was evaluated by histological examination and micro computed tomography (µ CT). The findings confirmed that constructed porous scaffolds consistently release exosomes; additionally, in vivo findings including Hematoxilin & Eosin staining, Immunohistochemistry, Masson's trichrome, histomorphometric analysis, and µ CT clarified that our implant has osteogenic properties. We discovered that Exo-treated scaffolds might promote osteogenesis especially compared to pure scaffolds, indicating that produced scaffolds containing exosomes could be a potential replacement in bone tissue engineering.
Bibliografia:ObjectType-Article-1
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ISSN:1389-9333
1573-6814
1573-6814
DOI:10.1007/s10561-023-10120-1