Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study

Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single‐stage and nested reverse transcription‐polymerase chain reaction (RT‐PCR) assays were developed that targeted the GPC and N...

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Bibliographic Details
Published in:	Journal of medical virology Vol. 51; no. 2; pp. 107 - 114
Main Authors:	Park, Jong Y., Peters, C. J., Rollin, Pierre E., Ksiazek, Tom G., Gray, Barry, Waites, Ken B., Stephensen, Charles B.
Format:	Journal Article
Language:	English
Published:	New York Wiley Subscription Services, Inc., A Wiley Company 01.02.1997 Wiley-Liss
Subjects:	Adult Antibodies, Viral - blood Antibodies, Viral - cerebrospinal fluid arenavirus Biological and medical sciences Child Child, Preschool encephalitis Enzyme-Linked Immunosorbent Assay Female Genes, Viral Human viral diseases Humans Infant Infant, Newborn Infectious diseases Lassa fever and arenovirus diseases Lymphocytic Choriomeningitis - cerebrospinal fluid Lymphocytic Choriomeningitis - diagnosis Lymphocytic Choriomeningitis - immunology lymphocytic choriomeningitis virus Lymphocytic choriomeningitis virus - isolation & purification Male Medical sciences meningitis Middle Aged Nucleocapsid - genetics Polymerase Chain Reaction - methods Population Surveillance Prospective Studies RNA, Viral - cerebrospinal fluid serum antibody Transcription, Genetic Tropical viral diseases Viral diseases Human Encephalitis Nervous system diseases RNA Lymphocytic choriomeningitis Reverse transcription Method Arenavirus Cerebral disorder Infection Virus Polymerase chain reaction Lymphocytic choriomeningitis virus Viral disease Central nervous system disease Meningitis Diagnosis Detection Arenaviridae
ISSN:	0146-6615, 1096-9071
Online Access:	Get full text
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