Development of a reverse transcription-polymerase chain reaction assay for diagnosis of lymphocytic choriomeningitis virus infection and its use in a prospective surveillance study

Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single‐stage and nested reverse transcription‐polymerase chain reaction (RT‐PCR) assays were developed that targeted the GPC and N...

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Vydáno v:	Journal of medical virology Ročník 51; číslo 2; s. 107 - 114
Hlavní autoři:	Park, Jong Y., Peters, C. J., Rollin, Pierre E., Ksiazek, Tom G., Gray, Barry, Waites, Ken B., Stephensen, Charles B.
Médium:	Journal Article
Jazyk:	angličtina
Vydáno:	New York Wiley Subscription Services, Inc., A Wiley Company 01.02.1997 Wiley-Liss
Témata:	Adult Antibodies, Viral - blood Antibodies, Viral - cerebrospinal fluid arenavirus Biological and medical sciences Child Child, Preschool encephalitis Enzyme-Linked Immunosorbent Assay Female Genes, Viral Human viral diseases Humans Infant Infant, Newborn Infectious diseases Lassa fever and arenovirus diseases Lymphocytic Choriomeningitis - cerebrospinal fluid Lymphocytic Choriomeningitis - diagnosis Lymphocytic Choriomeningitis - immunology lymphocytic choriomeningitis virus Lymphocytic choriomeningitis virus - isolation & purification Male Medical sciences meningitis Middle Aged Nucleocapsid - genetics Polymerase Chain Reaction - methods Population Surveillance Prospective Studies RNA, Viral - cerebrospinal fluid serum antibody Transcription, Genetic Tropical viral diseases Viral diseases Human Encephalitis Nervous system diseases RNA Lymphocytic choriomeningitis Reverse transcription Method Arenavirus Cerebral disorder Infection Virus Polymerase chain reaction Lymphocytic choriomeningitis virus Viral disease Central nervous system disease Meningitis Diagnosis Detection Arenaviridae
ISSN:	0146-6615, 1096-9071
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Shrnutí:	Lymphocytic choriomeningitis virus (LCMV), which is one of several arenaviruses that are pathogenic for humans, causes encephalitis and meningitis in man. In this study, single‐stage and nested reverse transcription‐polymerase chain reaction (RT‐PCR) assays were developed that targeted the GPC and N genes of LCMV. Both assays detected <1 TCID50 unit of LCMV. These assays were used to measure the incidence of LCMV infection by testing cerebrospinal fluid (CSF) samples with ⩾10 leukocytes/μI collected over 1 year from patients undergoing lumbar puncture for diagnostic reasons at two Birmingham hospitals. Samples were tested for the presence of LCMV RNA by using the RT‐PCR assay and for LCMV‐specific IgM antibody by using an ELISA assay. None of the specimens collected from 813 patients was positive by either assay. Although no cases of acute infection were detected, 4% (11/272) of serum collected from a subset of patients was positive for LCMV‐specific IgG. A significantly greater rate of seropositivity was found among subjects over 60 years of age (9.4%; P < 0.025) than was found in younger subjects (2.4% at 30–59 years of age; 0% at <30 years of age). These data suggest that serious central nervous system disease due to LCMV infection is not common in this population. The high rate of seropositivity in those over 60 years of age suggest that infection was once more common. J Med Virol 51:107–114, 1997. © 1997 Wiley‐Liss, Inc.
Bibliografie:	istex:A29C29DC30AB8FA6468E9B70CF3FFD4D737A2E81 Public Health Service - No. P40 RR00463 ArticleID:JMV4 ark:/67375/WNG-W4SXWLB9-V ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0146-6615 1096-9071
DOI:	10.1002/(SICI)1096-9071(199702)51:2<107::AID-JMV4>3.0.CO;2-B