Esomeprazole inhibits endoplasmic reticulum stress and ameliorates myocardial ischemia-reperfusion injury

Proton pump inhibitors (PPIs) are often prescribed in association with clopidogrel and aspirin to patients with myocardial infraction (MI), but their effects on heart is controversial. The purpose of this study was to investigate the effects and potential mechanism of omeprazole (OME) and esomeprazo...

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Vydané v:Biochemical and biophysical research communications Ročník 627; s. 84 - 90
Hlavní autori: Zhou, Guoxiang, Peng, Yuce, Guo, Mingyu, Qu, Can, Luo, Suxin, Jiang, Yingjiu, Chen, Dan, Wang, Xiaowen, Guo, Yongzheng
Médium: Journal Article
Jazyk:English
Vydavateľské údaje: Elsevier Inc 30.10.2022
Predmet:
aspirin
blood serum
cardiac output
cardiomyocytes
echocardiography
Endoplasmic reticulum stress
eosin
immunohistochemistry
Inflammation
Ischemia reperfusion (I/R) injury
macrophages
myocardial ischemia
proton pump
Proton pump inhibitors
surgery
Western blotting
Proton pump inhibitors
Inflammation
Ischemia reperfusion (I/R) injury
Endoplasmic reticulum stress
ISSN:0006-291X, 1090-2104, 1090-2104
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Shrnutí:Proton pump inhibitors (PPIs) are often prescribed in association with clopidogrel and aspirin to patients with myocardial infraction (MI), but their effects on heart is controversial. The purpose of this study was to investigate the effects and potential mechanism of omeprazole (OME) and esomeprazole (ESO) in myocardial ischemia reperfusion (I/R) injury. In the present study, mice were treated with OME, ESO or vehicle for 3 weeks and then subjected to myocardial I/R or sham surgery. At 1 day after surgery, echocardiography was performed to access cardiac injury. Hematoxylin and eosin (H&E) staining was performed to evaluate cardiomyocyte morphology. The IL1β was evaluated by Immunohistochemistry (IHC). Elisa was used to detect cTnt content in serum. The expression of CD86, CD206, CHOP, ATF6, eIF2α and p eIF2α were determined by Western blot (WB). The result showed that ESO markedly improved the left ventricular ejection fraction (LVEF), shortening fraction (FS), suppressed inflammatory infiltration, endoplasmic reticulum stress (ERS) and decreased proinflammatory macrophages in I/R hearts, while OME had no significant effects on cardiac function, inflammation and ERS in the I/R heart. In conclusion, ESO but not OME pretreatment reduces the proportion of proinflammatory macrophages, inhibits endoplasmic reticulum stress, and alleviates I/R injury in mice, indicating that ESO maybe a more proper PPI than OME for application in I/R injury. •Compared with omeprazole, esomeprazole pretreatment could alleviate ischemia-reperfusioninjury and improve cardiac function in mice.•Esomeprazole reduced proinflammatory macrophages and inhibits endoplasmic reticulum stress in the ischemia-reperfusion heart.
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ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2022.08.013